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Processing human skin samples for single-cell assays

Characterizing resident immune cells in human skin using single-cell assays provides insight into their role in infections, inflammation, and cancer. We describe an optimized protocol to rapidly isolate viable cells from 6-mm skin punch-biopsies. We provide an example in which we coupled single-cell...

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Detalles Bibliográficos
Autores principales:	Saluzzo, Simona, Gail, Laura Marie, Neuwirth, Teresa, Pandey, Ram Vinay, Farlik, Matthias, Stary, Georg
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234085/ https://www.ncbi.nlm.nih.gov/pubmed/35769924 http://dx.doi.org/10.1016/j.xpro.2022.101470

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