Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids

The ureteric bud (UB) is a kidney precursor tissue that repeats branching morphogenesis and gives rise to the collecting ducts (CDs) and lower urinary tract. Here, we describe protocols to generate iUB organoids from human iPSCs; iUB organoids repeat branching morphogenesis. We describe how to expan...

Descripción completa

Detalles Bibliográficos
Autores principales: Ryosaka, Makoto, Mae, Shin-Ichi, Osafune, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234152/
https://www.ncbi.nlm.nih.gov/pubmed/35769929
http://dx.doi.org/10.1016/j.xpro.2022.101484
_version_ 1784735993922846720
author Ryosaka, Makoto
Mae, Shin-Ichi
Osafune, Kenji
author_facet Ryosaka, Makoto
Mae, Shin-Ichi
Osafune, Kenji
author_sort Ryosaka, Makoto
collection PubMed
description The ureteric bud (UB) is a kidney precursor tissue that repeats branching morphogenesis and gives rise to the collecting ducts (CDs) and lower urinary tract. Here, we describe protocols to generate iUB organoids from human iPSCs; iUB organoids repeat branching morphogenesis. We describe how to expand iUB-organoid-derived tip colonies and how to induce CD progenitors from iUB organoids. These organoids can be used to study CD development and potentially as a model of kidney and urinary tract diseases. For complete details on the use and execution of this protocol, please refer to Mae et al. (2020).
format Online
Article
Text
id pubmed-9234152
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92341522022-06-28 Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids Ryosaka, Makoto Mae, Shin-Ichi Osafune, Kenji STAR Protoc Protocol The ureteric bud (UB) is a kidney precursor tissue that repeats branching morphogenesis and gives rise to the collecting ducts (CDs) and lower urinary tract. Here, we describe protocols to generate iUB organoids from human iPSCs; iUB organoids repeat branching morphogenesis. We describe how to expand iUB-organoid-derived tip colonies and how to induce CD progenitors from iUB organoids. These organoids can be used to study CD development and potentially as a model of kidney and urinary tract diseases. For complete details on the use and execution of this protocol, please refer to Mae et al. (2020). Elsevier 2022-06-17 /pmc/articles/PMC9234152/ /pubmed/35769929 http://dx.doi.org/10.1016/j.xpro.2022.101484 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Ryosaka, Makoto
Mae, Shin-Ichi
Osafune, Kenji
Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
title Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
title_full Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
title_fullStr Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
title_full_unstemmed Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
title_short Protocol for the generation and expansion of human iPS cell-derived ureteric bud organoids
title_sort protocol for the generation and expansion of human ips cell-derived ureteric bud organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234152/
https://www.ncbi.nlm.nih.gov/pubmed/35769929
http://dx.doi.org/10.1016/j.xpro.2022.101484
work_keys_str_mv AT ryosakamakoto protocolforthegenerationandexpansionofhumanipscellderiveduretericbudorganoids
AT maeshinichi protocolforthegenerationandexpansionofhumanipscellderiveduretericbudorganoids
AT osafunekenji protocolforthegenerationandexpansionofhumanipscellderiveduretericbudorganoids

Ejemplares similares