Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues

Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFA...

Descripción completa

Detalles Bibliográficos
Autores principales: Saçma, Mehmet, Matteini, Francesca, Mulaw, Medhanie A., Hageb, Ali, Bogeska, Ruzhica, Sakk, Vadim, Vollmer, Angelika, Marka, Gina, Soller, Karin, Milsom, Michael D., Florian, Maria Carolina, Geiger, Hartmut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234157/
https://www.ncbi.nlm.nih.gov/pubmed/35769923
http://dx.doi.org/10.1016/j.xpro.2022.101483
Descripción
Sumario:Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFAST3D) that preserves the 3D structure of the entire tissue and that of subcellular structures with high fidelity. The iFAST3D protocol enables reproducible and high-resolution 3D imaging of stem cells and various niche components for many mouse organs and tissues. For complete details on the use and execution of this protocol, please refer to Saçma et al. (2019).

Ejemplares similares