Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues

Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFA...

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Autores principales: Saçma, Mehmet, Matteini, Francesca, Mulaw, Medhanie A., Hageb, Ali, Bogeska, Ruzhica, Sakk, Vadim, Vollmer, Angelika, Marka, Gina, Soller, Karin, Milsom, Michael D., Florian, Maria Carolina, Geiger, Hartmut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234157/
https://www.ncbi.nlm.nih.gov/pubmed/35769923
http://dx.doi.org/10.1016/j.xpro.2022.101483
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author Saçma, Mehmet
Matteini, Francesca
Mulaw, Medhanie A.
Hageb, Ali
Bogeska, Ruzhica
Sakk, Vadim
Vollmer, Angelika
Marka, Gina
Soller, Karin
Milsom, Michael D.
Florian, Maria Carolina
Geiger, Hartmut
author_facet Saçma, Mehmet
Matteini, Francesca
Mulaw, Medhanie A.
Hageb, Ali
Bogeska, Ruzhica
Sakk, Vadim
Vollmer, Angelika
Marka, Gina
Soller, Karin
Milsom, Michael D.
Florian, Maria Carolina
Geiger, Hartmut
author_sort Saçma, Mehmet
collection PubMed
description Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFAST3D) that preserves the 3D structure of the entire tissue and that of subcellular structures with high fidelity. The iFAST3D protocol enables reproducible and high-resolution 3D imaging of stem cells and various niche components for many mouse organs and tissues. For complete details on the use and execution of this protocol, please refer to Saçma et al. (2019).
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spelling pubmed-92341572022-06-28 Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues Saçma, Mehmet Matteini, Francesca Mulaw, Medhanie A. Hageb, Ali Bogeska, Ruzhica Sakk, Vadim Vollmer, Angelika Marka, Gina Soller, Karin Milsom, Michael D. Florian, Maria Carolina Geiger, Hartmut STAR Protoc Protocol Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFAST3D) that preserves the 3D structure of the entire tissue and that of subcellular structures with high fidelity. The iFAST3D protocol enables reproducible and high-resolution 3D imaging of stem cells and various niche components for many mouse organs and tissues. For complete details on the use and execution of this protocol, please refer to Saçma et al. (2019). Elsevier 2022-06-17 /pmc/articles/PMC9234157/ /pubmed/35769923 http://dx.doi.org/10.1016/j.xpro.2022.101483 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Saçma, Mehmet
Matteini, Francesca
Mulaw, Medhanie A.
Hageb, Ali
Bogeska, Ruzhica
Sakk, Vadim
Vollmer, Angelika
Marka, Gina
Soller, Karin
Milsom, Michael D.
Florian, Maria Carolina
Geiger, Hartmut
Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
title Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
title_full Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
title_fullStr Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
title_full_unstemmed Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
title_short Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues
title_sort fast and high-fidelity in situ 3d imaging protocol for stem cells and niche components for mouse organs and tissues
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234157/
https://www.ncbi.nlm.nih.gov/pubmed/35769923
http://dx.doi.org/10.1016/j.xpro.2022.101483
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