Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues

Quantitative 3D imaging of organ-wide cellular and subcellular components is central for revealing and understanding complex interactions between stem cells and their microenvironment. Here, we present a gentle but fast whole-mount immunofluorescence staining protocol for 3D confocal microscopy (iFA...

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Detalles Bibliográficos
Autores principales: Saçma, Mehmet, Matteini, Francesca, Mulaw, Medhanie A., Hageb, Ali, Bogeska, Ruzhica, Sakk, Vadim, Vollmer, Angelika, Marka, Gina, Soller, Karin, Milsom, Michael D., Florian, Maria Carolina, Geiger, Hartmut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234157/
https://www.ncbi.nlm.nih.gov/pubmed/35769923
http://dx.doi.org/10.1016/j.xpro.2022.101483

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