Dataset of response characteristics of H(2)-producing bacteria consortium to β-lactams, aminoglycosides, macrolides, quinolones antibiotics

Antibiotics on H(2) producing bacteria shall be considered as being one of the critical elements in biological H(2) production utilizing livestock manure as raw resources. Despite the fact that the manure stands a significance role in bio-fermentation, the possibility of antibiotics being contained in excreta shall not be eliminated. Findings of whether the above saying might threaten the safety of bio-H(2) production needs to be further studied. The experiment subjects include: six single and three combined antibiotics were tested and analyzed by the application of the gradient experiment method. Along with the H(2) production rate, CHO content, pH and OD(600) were used to analyze the effects of various antibiotics introduction on the hydrolysis, fermentation and H(2) production. To a further extent, four typical representative samples were selected for biodiversity analysis from the single antibiotic experiment groups. Amounting more than 6000 pieces of data were obtained in a series of experiments. Data suggested that remarkable measure of antibiotics have various degrees of H(2) production inhibition, while some antibiotics, Penicillin G, Streptomycin Sulfate, and their compound antibiotics, could promote the growth of Ethanoligenens sp. and improve H(2) yield in the contrary. Correspondent to the transition of key metabolic intermediates and end products, the mechanism of each antibiotic type and dose on H(2) production were summarized as follows: the main inhibitory mechanisms were: (1) board-spectrum inhibition, (2) partial inhibition, (3) H(2) consumption enhancement; and the enhancement mechanisms were: (1) enhance the growth of H(2)-producing bacteria, (2) enhanced starch hydrolysis, (3) inhibitory H(2) consumption or release of acid inhibition. Meanwhile, data analysis found that the effect of antibiotics on H(2) producing was not only related to type, but also to dosage. Even one kind of antibiotic may have completely opposite effects on H(2)-producing bacteria under different dosage conditions. Inhibition of H(2) yield was highest with Levofloxacin at 6.15 mg/L, gas production was reduced by 88.77%; and enhancement of H(2) yield was highest with Penicillin G at 7.20 mg/L, the gas production increased by 72.90%.