Establishing a multiplex imaging panel to study T cell development in the thymus in mouse

Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage...

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Detalles Bibliográficos
Autores principales: Allam, Amr H., Russell, Sarah M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234470/
https://www.ncbi.nlm.nih.gov/pubmed/35769925
http://dx.doi.org/10.1016/j.xpro.2022.101472
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author Allam, Amr H.
Russell, Sarah M.
author_facet Allam, Amr H.
Russell, Sarah M.
author_sort Allam, Amr H.
collection PubMed
description Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).
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spelling pubmed-92344702022-06-28 Establishing a multiplex imaging panel to study T cell development in the thymus in mouse Allam, Amr H. Russell, Sarah M. STAR Protoc Protocol Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021). Elsevier 2022-06-21 /pmc/articles/PMC9234470/ /pubmed/35769925 http://dx.doi.org/10.1016/j.xpro.2022.101472 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Allam, Amr H.
Russell, Sarah M.
Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
title Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
title_full Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
title_fullStr Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
title_full_unstemmed Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
title_short Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
title_sort establishing a multiplex imaging panel to study t cell development in the thymus in mouse
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234470/
https://www.ncbi.nlm.nih.gov/pubmed/35769925
http://dx.doi.org/10.1016/j.xpro.2022.101472
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