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Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage...
Autores principales: | Allam, Amr H., Russell, Sarah M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234470/ https://www.ncbi.nlm.nih.gov/pubmed/35769925 http://dx.doi.org/10.1016/j.xpro.2022.101472 |
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