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Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection

Phytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native to Asia, which are generally healthy d...

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Autores principales: Saiz-Fernández, Iñigo, Đorđević, Biljana, Kerchev, Pavel, Černý, Martin, Jung, Thomas, Berka, Miroslav, Fu, Chuen-Hsu, Horta Jung, Marília, Brzobohatý, Břetislav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234522/
https://www.ncbi.nlm.nih.gov/pubmed/35770156
http://dx.doi.org/10.3389/fmicb.2022.894533
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author Saiz-Fernández, Iñigo
Đorđević, Biljana
Kerchev, Pavel
Černý, Martin
Jung, Thomas
Berka, Miroslav
Fu, Chuen-Hsu
Horta Jung, Marília
Brzobohatý, Břetislav
author_facet Saiz-Fernández, Iñigo
Đorđević, Biljana
Kerchev, Pavel
Černý, Martin
Jung, Thomas
Berka, Miroslav
Fu, Chuen-Hsu
Horta Jung, Marília
Brzobohatý, Břetislav
author_sort Saiz-Fernández, Iñigo
collection PubMed
description Phytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native to Asia, which are generally healthy despite the presence of the pathogen, the populations of Cork oaks (Quercus suber L.) in Europe have been severely decimated by P. cinnamomi. The present study aims at identifying the differences in the early proteomic and metabolomic response of these two tree species that lead to their differences in susceptibility to P. cinnamomi. By using micropropagated clonal plants, we tried to minimize the plant-to-plant differences in the defense response that is maximized by the high intraspecific genetic variability inherent to the Quercus genus. The evolution on the content of Phytophthora proteins in the roots during the first 36 h after inoculation suggests a slower infection process in Q. variabilis plants. These plants displayed a significant decrease in sugars in the roots, together with a downregulation of proteins related to carbon metabolism. In the leaves, the biggest changes in proteomic profiling were observed 16 h after inoculation, and included increased abundance of peroxidases, superoxide dismutases and glutathione S-transferases in Q. variabilis plants, which probably contributed to decrease its susceptibility to P. cinnamomi.
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spelling pubmed-92345222022-06-28 Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection Saiz-Fernández, Iñigo Đorđević, Biljana Kerchev, Pavel Černý, Martin Jung, Thomas Berka, Miroslav Fu, Chuen-Hsu Horta Jung, Marília Brzobohatý, Břetislav Front Microbiol Microbiology Phytophthora cinnamomi Rands is a cosmopolite pathogen of woody plants which during the last couple of centuries has spread all over the world from its center of origin in Southeast Asia. In contrast to Chinese cork oak (Quercus variabilis Blume) forests native to Asia, which are generally healthy despite the presence of the pathogen, the populations of Cork oaks (Quercus suber L.) in Europe have been severely decimated by P. cinnamomi. The present study aims at identifying the differences in the early proteomic and metabolomic response of these two tree species that lead to their differences in susceptibility to P. cinnamomi. By using micropropagated clonal plants, we tried to minimize the plant-to-plant differences in the defense response that is maximized by the high intraspecific genetic variability inherent to the Quercus genus. The evolution on the content of Phytophthora proteins in the roots during the first 36 h after inoculation suggests a slower infection process in Q. variabilis plants. These plants displayed a significant decrease in sugars in the roots, together with a downregulation of proteins related to carbon metabolism. In the leaves, the biggest changes in proteomic profiling were observed 16 h after inoculation, and included increased abundance of peroxidases, superoxide dismutases and glutathione S-transferases in Q. variabilis plants, which probably contributed to decrease its susceptibility to P. cinnamomi. Frontiers Media S.A. 2022-06-13 /pmc/articles/PMC9234522/ /pubmed/35770156 http://dx.doi.org/10.3389/fmicb.2022.894533 Text en Copyright © 2022 Saiz-Fernández, Đorđević, Kerchev, Černý, Jung, Berka, Fu, Horta Jung and Brzobohatý. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Saiz-Fernández, Iñigo
Đorđević, Biljana
Kerchev, Pavel
Černý, Martin
Jung, Thomas
Berka, Miroslav
Fu, Chuen-Hsu
Horta Jung, Marília
Brzobohatý, Břetislav
Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
title Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
title_full Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
title_fullStr Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
title_full_unstemmed Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
title_short Differences in the Proteomic and Metabolomic Response of Quercus suber and Quercus variabilis During the Early Stages of Phytophthora cinnamomi Infection
title_sort differences in the proteomic and metabolomic response of quercus suber and quercus variabilis during the early stages of phytophthora cinnamomi infection
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234522/
https://www.ncbi.nlm.nih.gov/pubmed/35770156
http://dx.doi.org/10.3389/fmicb.2022.894533
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