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Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids

Effective utilization of genetic diversity in wild relatives to improve wheat requires recombination between wheat and alien chromosomes. However, this is suppressed by the Pairing homoeologous gene, Ph1, on the long arm of wheat chromosome 5B. A deletion mutant of the Ph1 locus (ph1b) has been used...

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Autores principales: Türkösi, Edina, Ivanizs, László, Farkas, András, Gaál, Eszter, Kruppa, Klaudia, Kovács, Péter, Szakács, Éva, Szőke-Pázsi, Kitti, Said, Mahmoud, Cápal, Petr, Griffiths, Simon, Doležel, Jaroslav, Molnár, István
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234525/
https://www.ncbi.nlm.nih.gov/pubmed/35769292
http://dx.doi.org/10.3389/fpls.2022.875676
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author Türkösi, Edina
Ivanizs, László
Farkas, András
Gaál, Eszter
Kruppa, Klaudia
Kovács, Péter
Szakács, Éva
Szőke-Pázsi, Kitti
Said, Mahmoud
Cápal, Petr
Griffiths, Simon
Doležel, Jaroslav
Molnár, István
author_facet Türkösi, Edina
Ivanizs, László
Farkas, András
Gaál, Eszter
Kruppa, Klaudia
Kovács, Péter
Szakács, Éva
Szőke-Pázsi, Kitti
Said, Mahmoud
Cápal, Petr
Griffiths, Simon
Doležel, Jaroslav
Molnár, István
author_sort Türkösi, Edina
collection PubMed
description Effective utilization of genetic diversity in wild relatives to improve wheat requires recombination between wheat and alien chromosomes. However, this is suppressed by the Pairing homoeologous gene, Ph1, on the long arm of wheat chromosome 5B. A deletion mutant of the Ph1 locus (ph1b) has been used widely to induce homoeologous recombination in wheat × alien hybrids. However, the original ph1b mutation, developed in Chinese Spring (CS) background has poor agronomic performance. Hence, alien introgression lines are first backcrossed with adapted wheat genotypes and after this step, alien chromosome segments are introduced into breeding lines. In this work, the ph1b mutation was transferred from two CSph1b mutants into winter wheat line Mv9kr1. Homozygous genotypes Mv9kr1 ph1b/ph1b exhibited improved plant and spike morphology compared to Chinese Spring. Flow cytometric chromosome analysis confirmed reduced DNA content of the mutant 5B chromosome in both wheat genotype relative to the wild type chromosome. The ph1b mutation in the Mv9kr1 genotype allowed wheat-alien chromosome pairing in meiosis of Mv9kr1ph1b_K × Aegilops biuncialis F(1) hybrids, predominantly with the M(b)-genome chromosomes of Aegilops relative to those of the U(b) genome. High frequency of wheat-Aegilops chromosome interactions resulted in rearranged chromosomes identified in the new Mv9kr1ph1b × Ae. Biuncialis amphiploids, making these lines valuable sources for alien introgressions. The new Mv9kr1ph1b mutant genotype is a unique resource to support alien introgression breeding of hexaploid wheat.
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spelling pubmed-92345252022-06-28 Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids Türkösi, Edina Ivanizs, László Farkas, András Gaál, Eszter Kruppa, Klaudia Kovács, Péter Szakács, Éva Szőke-Pázsi, Kitti Said, Mahmoud Cápal, Petr Griffiths, Simon Doležel, Jaroslav Molnár, István Front Plant Sci Plant Science Effective utilization of genetic diversity in wild relatives to improve wheat requires recombination between wheat and alien chromosomes. However, this is suppressed by the Pairing homoeologous gene, Ph1, on the long arm of wheat chromosome 5B. A deletion mutant of the Ph1 locus (ph1b) has been used widely to induce homoeologous recombination in wheat × alien hybrids. However, the original ph1b mutation, developed in Chinese Spring (CS) background has poor agronomic performance. Hence, alien introgression lines are first backcrossed with adapted wheat genotypes and after this step, alien chromosome segments are introduced into breeding lines. In this work, the ph1b mutation was transferred from two CSph1b mutants into winter wheat line Mv9kr1. Homozygous genotypes Mv9kr1 ph1b/ph1b exhibited improved plant and spike morphology compared to Chinese Spring. Flow cytometric chromosome analysis confirmed reduced DNA content of the mutant 5B chromosome in both wheat genotype relative to the wild type chromosome. The ph1b mutation in the Mv9kr1 genotype allowed wheat-alien chromosome pairing in meiosis of Mv9kr1ph1b_K × Aegilops biuncialis F(1) hybrids, predominantly with the M(b)-genome chromosomes of Aegilops relative to those of the U(b) genome. High frequency of wheat-Aegilops chromosome interactions resulted in rearranged chromosomes identified in the new Mv9kr1ph1b × Ae. Biuncialis amphiploids, making these lines valuable sources for alien introgressions. The new Mv9kr1ph1b mutant genotype is a unique resource to support alien introgression breeding of hexaploid wheat. Frontiers Media S.A. 2022-06-13 /pmc/articles/PMC9234525/ /pubmed/35769292 http://dx.doi.org/10.3389/fpls.2022.875676 Text en Copyright © 2022 Türkösi, Ivanizs, Farkas, Gaál, Kruppa, Kovács, Szakács, Szőke-Pázsi, Said, Cápal, Griffiths, Doležel and Molnár. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Türkösi, Edina
Ivanizs, László
Farkas, András
Gaál, Eszter
Kruppa, Klaudia
Kovács, Péter
Szakács, Éva
Szőke-Pázsi, Kitti
Said, Mahmoud
Cápal, Petr
Griffiths, Simon
Doležel, Jaroslav
Molnár, István
Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids
title Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids
title_full Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids
title_fullStr Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids
title_full_unstemmed Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids
title_short Transfer of the ph1b Deletion Chromosome 5B From Chinese Spring Wheat Into a Winter Wheat Line and Induction of Chromosome Rearrangements in Wheat-Aegilops biuncialis Hybrids
title_sort transfer of the ph1b deletion chromosome 5b from chinese spring wheat into a winter wheat line and induction of chromosome rearrangements in wheat-aegilops biuncialis hybrids
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234525/
https://www.ncbi.nlm.nih.gov/pubmed/35769292
http://dx.doi.org/10.3389/fpls.2022.875676
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