Cargando…

The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling

Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal effectors regulate plant defense is of great importance for the development of novel strategies for disease control. In this study, we identified necrosis- and ethylene-inducing...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Guangyong, Yang, Jie, Zhang, Qiwei, Wang, Wenfeng, Feng, Liping, Zhao, Li, An, Bang, Wang, Qiannan, He, Chaozu, Luo, Hongli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234567/
https://www.ncbi.nlm.nih.gov/pubmed/35770165
http://dx.doi.org/10.3389/fmicb.2022.911479
_version_ 1784736106983456768
author Yang, Guangyong
Yang, Jie
Zhang, Qiwei
Wang, Wenfeng
Feng, Liping
Zhao, Li
An, Bang
Wang, Qiannan
He, Chaozu
Luo, Hongli
author_facet Yang, Guangyong
Yang, Jie
Zhang, Qiwei
Wang, Wenfeng
Feng, Liping
Zhao, Li
An, Bang
Wang, Qiannan
He, Chaozu
Luo, Hongli
author_sort Yang, Guangyong
collection PubMed
description Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal effectors regulate plant defense is of great importance for the development of novel strategies for disease control. In this study, we identified necrosis- and ethylene-inducing protein 1 (Nep1)-like protein (NLP) effector gene, CgNLP1, which contributed to conidial germination, appressorium formation, invasive growth, and virulence of Colletotrichum gloeosporioides to the rubber tree. Transient expression of CgNLP1 in the leaves of Nicotiana benthamiana induced ethylene production in plants. Ectopic expression of CgNLP1 in Arabidopsis significantly enhanced the resistance to Botrytis cinerea and Alternaria brassicicola. An R2R3 type transcription factor HbMYB8-like of rubber tree was identified as the target of CgNLP1.HbMYB8-like, localized on the nucleus, and induced cell death in N. benthamiana. CgNLP1 disrupted nuclear accumulation of HbMYB8-like and suppressed HbMYB8-like induced cell death, which is mediated by the salicylic acid (SA) signal pathway. This study suggested a new strategy whereby C. gloeosporioides exploited the CgNLP1 effector to affect invasion and suppress a host defense regulator HbMYB8-like to facilitate infection.
format Online
Article
Text
id pubmed-9234567
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-92345672022-06-28 The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling Yang, Guangyong Yang, Jie Zhang, Qiwei Wang, Wenfeng Feng, Liping Zhao, Li An, Bang Wang, Qiannan He, Chaozu Luo, Hongli Front Microbiol Microbiology Fungi secrete numerous effectors to modulate host defense systems. Understanding the molecular mechanisms by which fungal effectors regulate plant defense is of great importance for the development of novel strategies for disease control. In this study, we identified necrosis- and ethylene-inducing protein 1 (Nep1)-like protein (NLP) effector gene, CgNLP1, which contributed to conidial germination, appressorium formation, invasive growth, and virulence of Colletotrichum gloeosporioides to the rubber tree. Transient expression of CgNLP1 in the leaves of Nicotiana benthamiana induced ethylene production in plants. Ectopic expression of CgNLP1 in Arabidopsis significantly enhanced the resistance to Botrytis cinerea and Alternaria brassicicola. An R2R3 type transcription factor HbMYB8-like of rubber tree was identified as the target of CgNLP1.HbMYB8-like, localized on the nucleus, and induced cell death in N. benthamiana. CgNLP1 disrupted nuclear accumulation of HbMYB8-like and suppressed HbMYB8-like induced cell death, which is mediated by the salicylic acid (SA) signal pathway. This study suggested a new strategy whereby C. gloeosporioides exploited the CgNLP1 effector to affect invasion and suppress a host defense regulator HbMYB8-like to facilitate infection. Frontiers Media S.A. 2022-06-13 /pmc/articles/PMC9234567/ /pubmed/35770165 http://dx.doi.org/10.3389/fmicb.2022.911479 Text en Copyright © 2022 Yang, Yang, Zhang, Wang, Feng, Zhao, An, Wang, He and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yang, Guangyong
Yang, Jie
Zhang, Qiwei
Wang, Wenfeng
Feng, Liping
Zhao, Li
An, Bang
Wang, Qiannan
He, Chaozu
Luo, Hongli
The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
title The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
title_full The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
title_fullStr The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
title_full_unstemmed The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
title_short The Effector Protein CgNLP1 of Colletotrichum gloeosporioides Affects Invasion and Disrupts Nuclear Localization of Necrosis-Induced Transcription Factor HbMYB8-Like to Suppress Plant Defense Signaling
title_sort effector protein cgnlp1 of colletotrichum gloeosporioides affects invasion and disrupts nuclear localization of necrosis-induced transcription factor hbmyb8-like to suppress plant defense signaling
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234567/
https://www.ncbi.nlm.nih.gov/pubmed/35770165
http://dx.doi.org/10.3389/fmicb.2022.911479
work_keys_str_mv AT yangguangyong theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT yangjie theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT zhangqiwei theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT wangwenfeng theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT fengliping theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT zhaoli theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT anbang theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT wangqiannan theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT hechaozu theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT luohongli theeffectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT yangguangyong effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT yangjie effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT zhangqiwei effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT wangwenfeng effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT fengliping effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT zhaoli effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT anbang effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT wangqiannan effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT hechaozu effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling
AT luohongli effectorproteincgnlp1ofcolletotrichumgloeosporioidesaffectsinvasionanddisruptsnuclearlocalizationofnecrosisinducedtranscriptionfactorhbmyb8liketosuppressplantdefensesignaling