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MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of mi...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Publication of Acta Dermato-Venereologica
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234987/ https://www.ncbi.nlm.nih.gov/pubmed/32556351 http://dx.doi.org/10.2340/00015555-3574 |
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author | LINDAHL, Lise M. GLUUD, Maria EMMANUEL, Thomas THOMSEN, Emil A. HU, Tengpeng RITTIG, Anne H. CELIS, Pamela STOLEARENCO, Veronica KREJSGAARD, Thorbjørn JOHANSEN, Claus WILLERSLEV-OLSEN, Andreas BUUS, Terkild B. WOETMANN, Anders AAGAARD, Lars GEISLER, Carsten LITMAN, Thomas MIKKELSEN, Jacob G. ODUM, Niels IVERSEN, Lars |
author_facet | LINDAHL, Lise M. GLUUD, Maria EMMANUEL, Thomas THOMSEN, Emil A. HU, Tengpeng RITTIG, Anne H. CELIS, Pamela STOLEARENCO, Veronica KREJSGAARD, Thorbjørn JOHANSEN, Claus WILLERSLEV-OLSEN, Andreas BUUS, Terkild B. WOETMANN, Anders AAGAARD, Lars GEISLER, Carsten LITMAN, Thomas MIKKELSEN, Jacob G. ODUM, Niels IVERSEN, Lars |
author_sort | LINDAHL, Lise M. |
collection | PubMed |
description | A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides. |
format | Online Article Text |
id | pubmed-9234987 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Society for Publication of Acta Dermato-Venereologica |
record_format | MEDLINE/PubMed |
spelling | pubmed-92349872022-10-20 MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides LINDAHL, Lise M. GLUUD, Maria EMMANUEL, Thomas THOMSEN, Emil A. HU, Tengpeng RITTIG, Anne H. CELIS, Pamela STOLEARENCO, Veronica KREJSGAARD, Thorbjørn JOHANSEN, Claus WILLERSLEV-OLSEN, Andreas BUUS, Terkild B. WOETMANN, Anders AAGAARD, Lars GEISLER, Carsten LITMAN, Thomas MIKKELSEN, Jacob G. ODUM, Niels IVERSEN, Lars Acta Derm Venereol Investigative Report A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides. Society for Publication of Acta Dermato-Venereologica 2020-09-30 /pmc/articles/PMC9234987/ /pubmed/32556351 http://dx.doi.org/10.2340/00015555-3574 Text en © 2020 Acta Dermato-Venereologica https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license |
spellingShingle | Investigative Report LINDAHL, Lise M. GLUUD, Maria EMMANUEL, Thomas THOMSEN, Emil A. HU, Tengpeng RITTIG, Anne H. CELIS, Pamela STOLEARENCO, Veronica KREJSGAARD, Thorbjørn JOHANSEN, Claus WILLERSLEV-OLSEN, Andreas BUUS, Terkild B. WOETMANN, Anders AAGAARD, Lars GEISLER, Carsten LITMAN, Thomas MIKKELSEN, Jacob G. ODUM, Niels IVERSEN, Lars MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides |
title | MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides |
title_full | MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides |
title_fullStr | MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides |
title_full_unstemmed | MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides |
title_short | MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides |
title_sort | microrna-106b regulates expression of the tumour suppressors p21 and txnip and promotes tumour cell proliferation in mycosis fungoides |
topic | Investigative Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234987/ https://www.ncbi.nlm.nih.gov/pubmed/32556351 http://dx.doi.org/10.2340/00015555-3574 |
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