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MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of mi...

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Autores principales: LINDAHL, Lise M., GLUUD, Maria, EMMANUEL, Thomas, THOMSEN, Emil A., HU, Tengpeng, RITTIG, Anne H., CELIS, Pamela, STOLEARENCO, Veronica, KREJSGAARD, Thorbjørn, JOHANSEN, Claus, WILLERSLEV-OLSEN, Andreas, BUUS, Terkild B., WOETMANN, Anders, AAGAARD, Lars, GEISLER, Carsten, LITMAN, Thomas, MIKKELSEN, Jacob G., ODUM, Niels, IVERSEN, Lars
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society for Publication of Acta Dermato-Venereologica 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234987/
https://www.ncbi.nlm.nih.gov/pubmed/32556351
http://dx.doi.org/10.2340/00015555-3574
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author LINDAHL, Lise M.
GLUUD, Maria
EMMANUEL, Thomas
THOMSEN, Emil A.
HU, Tengpeng
RITTIG, Anne H.
CELIS, Pamela
STOLEARENCO, Veronica
KREJSGAARD, Thorbjørn
JOHANSEN, Claus
WILLERSLEV-OLSEN, Andreas
BUUS, Terkild B.
WOETMANN, Anders
AAGAARD, Lars
GEISLER, Carsten
LITMAN, Thomas
MIKKELSEN, Jacob G.
ODUM, Niels
IVERSEN, Lars
author_facet LINDAHL, Lise M.
GLUUD, Maria
EMMANUEL, Thomas
THOMSEN, Emil A.
HU, Tengpeng
RITTIG, Anne H.
CELIS, Pamela
STOLEARENCO, Veronica
KREJSGAARD, Thorbjørn
JOHANSEN, Claus
WILLERSLEV-OLSEN, Andreas
BUUS, Terkild B.
WOETMANN, Anders
AAGAARD, Lars
GEISLER, Carsten
LITMAN, Thomas
MIKKELSEN, Jacob G.
ODUM, Niels
IVERSEN, Lars
author_sort LINDAHL, Lise M.
collection PubMed
description A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.
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spelling pubmed-92349872022-10-20 MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides LINDAHL, Lise M. GLUUD, Maria EMMANUEL, Thomas THOMSEN, Emil A. HU, Tengpeng RITTIG, Anne H. CELIS, Pamela STOLEARENCO, Veronica KREJSGAARD, Thorbjørn JOHANSEN, Claus WILLERSLEV-OLSEN, Andreas BUUS, Terkild B. WOETMANN, Anders AAGAARD, Lars GEISLER, Carsten LITMAN, Thomas MIKKELSEN, Jacob G. ODUM, Niels IVERSEN, Lars Acta Derm Venereol Investigative Report A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides. Society for Publication of Acta Dermato-Venereologica 2020-09-30 /pmc/articles/PMC9234987/ /pubmed/32556351 http://dx.doi.org/10.2340/00015555-3574 Text en © 2020 Acta Dermato-Venereologica https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the CC BY-NC license
spellingShingle Investigative Report
LINDAHL, Lise M.
GLUUD, Maria
EMMANUEL, Thomas
THOMSEN, Emil A.
HU, Tengpeng
RITTIG, Anne H.
CELIS, Pamela
STOLEARENCO, Veronica
KREJSGAARD, Thorbjørn
JOHANSEN, Claus
WILLERSLEV-OLSEN, Andreas
BUUS, Terkild B.
WOETMANN, Anders
AAGAARD, Lars
GEISLER, Carsten
LITMAN, Thomas
MIKKELSEN, Jacob G.
ODUM, Niels
IVERSEN, Lars
MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
title MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
title_full MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
title_fullStr MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
title_full_unstemmed MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
title_short MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides
title_sort microrna-106b regulates expression of the tumour suppressors p21 and txnip and promotes tumour cell proliferation in mycosis fungoides
topic Investigative Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234987/
https://www.ncbi.nlm.nih.gov/pubmed/32556351
http://dx.doi.org/10.2340/00015555-3574
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