Cargando…
Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application
Photoluminescent nanomaterials have been widely employed in several biological applications both in vitro and in vivo. For the first time, we report a novel application of sour apple-derived photoluminescent carbon dots (PCDs) for reducing ultra-high molecular weight polyethylene (UHMWPE) wear parti...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235131/ https://www.ncbi.nlm.nih.gov/pubmed/35761350 http://dx.doi.org/10.1186/s12951-022-01498-3 |
_version_ | 1784736241335402496 |
---|---|
author | Li, Xiang Lu, Yang Li, Jiarui Zhou, Shengji Wang, Yuxin Li, Liangping Zhao, Fengchao |
author_facet | Li, Xiang Lu, Yang Li, Jiarui Zhou, Shengji Wang, Yuxin Li, Liangping Zhao, Fengchao |
author_sort | Li, Xiang |
collection | PubMed |
description | Photoluminescent nanomaterials have been widely employed in several biological applications both in vitro and in vivo. For the first time, we report a novel application of sour apple-derived photoluminescent carbon dots (PCDs) for reducing ultra-high molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis using mouse calvarial model. Generally, aseptic prosthetic loosening seems to be a significant postoperative problem for artificial joints replacement, which is mainly contributed by UHMWPE-induced osteolysis. Hence, inhibiting osteoclastic bone-resorption could minimize UHMWPE-induced osteolysis for implant loosening. Prior to osteolysis studies, the prepared sour apple-derived PCDs were employed for bioimaging application. As expected, the prepared PCDs effectively inhibited the UHMWPE particle-induced osteoclastogenesis in vitro. The PCDs treatment effectively inhibited the UHMWPE-induced osteoclast differentiation, F-actin ring pattern, and bone resorption in vitro. Also, the PCDs reduced the UHMWPE-induced ROS stress as well as the expression level of pro-inflammatory cytokines, including TNF-α, IL-1, IL-6, and IL-8. Further, the qPCR and western blot results hypothesized that PCDs inhibited the UHMWPE wear particle-induced osteolysis through suppressing chemerin/ChemR23 signaling and NFATc1 pathway, along with upregulation of SIRT1 expression. Overall, these findings suggest that the synthesized PCDs could be a potential therapeutic material for minimizing UHMWPE particle-induced periprosthetic osteolysis to avoid postoperative complications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01498-3. |
format | Online Article Text |
id | pubmed-9235131 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92351312022-06-28 Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application Li, Xiang Lu, Yang Li, Jiarui Zhou, Shengji Wang, Yuxin Li, Liangping Zhao, Fengchao J Nanobiotechnology Research Photoluminescent nanomaterials have been widely employed in several biological applications both in vitro and in vivo. For the first time, we report a novel application of sour apple-derived photoluminescent carbon dots (PCDs) for reducing ultra-high molecular weight polyethylene (UHMWPE) wear particle-induced osteolysis using mouse calvarial model. Generally, aseptic prosthetic loosening seems to be a significant postoperative problem for artificial joints replacement, which is mainly contributed by UHMWPE-induced osteolysis. Hence, inhibiting osteoclastic bone-resorption could minimize UHMWPE-induced osteolysis for implant loosening. Prior to osteolysis studies, the prepared sour apple-derived PCDs were employed for bioimaging application. As expected, the prepared PCDs effectively inhibited the UHMWPE particle-induced osteoclastogenesis in vitro. The PCDs treatment effectively inhibited the UHMWPE-induced osteoclast differentiation, F-actin ring pattern, and bone resorption in vitro. Also, the PCDs reduced the UHMWPE-induced ROS stress as well as the expression level of pro-inflammatory cytokines, including TNF-α, IL-1, IL-6, and IL-8. Further, the qPCR and western blot results hypothesized that PCDs inhibited the UHMWPE wear particle-induced osteolysis through suppressing chemerin/ChemR23 signaling and NFATc1 pathway, along with upregulation of SIRT1 expression. Overall, these findings suggest that the synthesized PCDs could be a potential therapeutic material for minimizing UHMWPE particle-induced periprosthetic osteolysis to avoid postoperative complications. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-022-01498-3. BioMed Central 2022-06-27 /pmc/articles/PMC9235131/ /pubmed/35761350 http://dx.doi.org/10.1186/s12951-022-01498-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Li, Xiang Lu, Yang Li, Jiarui Zhou, Shengji Wang, Yuxin Li, Liangping Zhao, Fengchao Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application |
title | Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application |
title_full | Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application |
title_fullStr | Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application |
title_full_unstemmed | Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application |
title_short | Photoluminescent carbon dots (PCDs) from sour apple: a biocompatible nanomaterial for preventing UHMWPE wear-particle induced osteolysis via modulating Chemerin/ChemR23 and SIRT1 signaling pathway and its bioimaging application |
title_sort | photoluminescent carbon dots (pcds) from sour apple: a biocompatible nanomaterial for preventing uhmwpe wear-particle induced osteolysis via modulating chemerin/chemr23 and sirt1 signaling pathway and its bioimaging application |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235131/ https://www.ncbi.nlm.nih.gov/pubmed/35761350 http://dx.doi.org/10.1186/s12951-022-01498-3 |
work_keys_str_mv | AT lixiang photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication AT luyang photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication AT lijiarui photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication AT zhoushengji photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication AT wangyuxin photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication AT liliangping photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication AT zhaofengchao photoluminescentcarbondotspcdsfromsourappleabiocompatiblenanomaterialforpreventinguhmwpewearparticleinducedosteolysisviamodulatingchemerinchemr23andsirt1signalingpathwayanditsbioimagingapplication |