Cargando…

Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase

BACKGROUND: Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1. METHODS: In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yuanzhi, Li, Yanglin, Wang, Mingshu, Cheng, Anchun, Ou, Xumin, Mao, Sai, Sun, Di, Wu, Ying, Yang, Qiao, Jia, Renyong, Tian, Bin, Zhang, Shaqiu, Zhu, Dekang, Chen, Shun, Liu, Mafeng, Zhao, Xinxin, Huang, Juan, Gao, Qun, Yu, Yanling, Zhang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235186/
https://www.ncbi.nlm.nih.gov/pubmed/35761382
http://dx.doi.org/10.1186/s12985-022-01839-6
Descripción
Sumario:BACKGROUND: Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1. METHODS: In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cell cycle of duck embryo fibroblasts (DEFs). Subsequently, we analyzed the effects of cell cycle phases on DHAV-1 replication by real-time reverse transcriptase quantitative PCR (real-time RT-qPCR). RESULTS: Flow cytometry data analysis found that DEFs in the S phase increased by 25.85% and 54.21% at 24 h and 48 h after DHAV-1 infection, respectively. The levels of viral RNA detected by real-time RT-qPCR were higher in the DEFs with synchronization in the S phase or G0/G1 phase than in the control group. However, there was no difference in viral copy number between the G2/M phase arrest and control groups. In addition, non-structural protein 3D of DHAV-1 significantly increased cells in the S phase, indicating that 3D protein is one of the reasons for the cell cycle arrest in the S phase. CONCLUSIONS: In summary, DHAV-1 infection induces the cell cycle arrest of DEFs in the S phase. Both S phase and G0/G1 phase synchronization facilitate the replication of DHAV-1, and 3D protein is one of the reasons for the S phase arrest.