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Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase

BACKGROUND: Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1. METHODS: In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cel...

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Autores principales: Liu, Yuanzhi, Li, Yanglin, Wang, Mingshu, Cheng, Anchun, Ou, Xumin, Mao, Sai, Sun, Di, Wu, Ying, Yang, Qiao, Jia, Renyong, Tian, Bin, Zhang, Shaqiu, Zhu, Dekang, Chen, Shun, Liu, Mafeng, Zhao, Xinxin, Huang, Juan, Gao, Qun, Yu, Yanling, Zhang, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235186/
https://www.ncbi.nlm.nih.gov/pubmed/35761382
http://dx.doi.org/10.1186/s12985-022-01839-6
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author Liu, Yuanzhi
Li, Yanglin
Wang, Mingshu
Cheng, Anchun
Ou, Xumin
Mao, Sai
Sun, Di
Wu, Ying
Yang, Qiao
Jia, Renyong
Tian, Bin
Zhang, Shaqiu
Zhu, Dekang
Chen, Shun
Liu, Mafeng
Zhao, Xinxin
Huang, Juan
Gao, Qun
Yu, Yanling
Zhang, Ling
author_facet Liu, Yuanzhi
Li, Yanglin
Wang, Mingshu
Cheng, Anchun
Ou, Xumin
Mao, Sai
Sun, Di
Wu, Ying
Yang, Qiao
Jia, Renyong
Tian, Bin
Zhang, Shaqiu
Zhu, Dekang
Chen, Shun
Liu, Mafeng
Zhao, Xinxin
Huang, Juan
Gao, Qun
Yu, Yanling
Zhang, Ling
author_sort Liu, Yuanzhi
collection PubMed
description BACKGROUND: Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1. METHODS: In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cell cycle of duck embryo fibroblasts (DEFs). Subsequently, we analyzed the effects of cell cycle phases on DHAV-1 replication by real-time reverse transcriptase quantitative PCR (real-time RT-qPCR). RESULTS: Flow cytometry data analysis found that DEFs in the S phase increased by 25.85% and 54.21% at 24 h and 48 h after DHAV-1 infection, respectively. The levels of viral RNA detected by real-time RT-qPCR were higher in the DEFs with synchronization in the S phase or G0/G1 phase than in the control group. However, there was no difference in viral copy number between the G2/M phase arrest and control groups. In addition, non-structural protein 3D of DHAV-1 significantly increased cells in the S phase, indicating that 3D protein is one of the reasons for the cell cycle arrest in the S phase. CONCLUSIONS: In summary, DHAV-1 infection induces the cell cycle arrest of DEFs in the S phase. Both S phase and G0/G1 phase synchronization facilitate the replication of DHAV-1, and 3D protein is one of the reasons for the S phase arrest.
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spelling pubmed-92351862022-06-28 Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase Liu, Yuanzhi Li, Yanglin Wang, Mingshu Cheng, Anchun Ou, Xumin Mao, Sai Sun, Di Wu, Ying Yang, Qiao Jia, Renyong Tian, Bin Zhang, Shaqiu Zhu, Dekang Chen, Shun Liu, Mafeng Zhao, Xinxin Huang, Juan Gao, Qun Yu, Yanling Zhang, Ling Virol J Research BACKGROUND: Duck hepatitis A virus type 1 (DHAV-1) is one of the most serious pathogens endangering the duck industry. However, there are few studies on the regulation of the cell cycle by DHAV-1. METHODS: In this study, flow cytometry was applied to analyze the effect of DHAV-1 infection on the cell cycle of duck embryo fibroblasts (DEFs). Subsequently, we analyzed the effects of cell cycle phases on DHAV-1 replication by real-time reverse transcriptase quantitative PCR (real-time RT-qPCR). RESULTS: Flow cytometry data analysis found that DEFs in the S phase increased by 25.85% and 54.21% at 24 h and 48 h after DHAV-1 infection, respectively. The levels of viral RNA detected by real-time RT-qPCR were higher in the DEFs with synchronization in the S phase or G0/G1 phase than in the control group. However, there was no difference in viral copy number between the G2/M phase arrest and control groups. In addition, non-structural protein 3D of DHAV-1 significantly increased cells in the S phase, indicating that 3D protein is one of the reasons for the cell cycle arrest in the S phase. CONCLUSIONS: In summary, DHAV-1 infection induces the cell cycle arrest of DEFs in the S phase. Both S phase and G0/G1 phase synchronization facilitate the replication of DHAV-1, and 3D protein is one of the reasons for the S phase arrest. BioMed Central 2022-06-27 /pmc/articles/PMC9235186/ /pubmed/35761382 http://dx.doi.org/10.1186/s12985-022-01839-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Yuanzhi
Li, Yanglin
Wang, Mingshu
Cheng, Anchun
Ou, Xumin
Mao, Sai
Sun, Di
Wu, Ying
Yang, Qiao
Jia, Renyong
Tian, Bin
Zhang, Shaqiu
Zhu, Dekang
Chen, Shun
Liu, Mafeng
Zhao, Xinxin
Huang, Juan
Gao, Qun
Yu, Yanling
Zhang, Ling
Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase
title Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase
title_full Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase
title_fullStr Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase
title_full_unstemmed Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase
title_short Duck hepatitis A virus type 1 mediates cell cycle arrest in the S phase
title_sort duck hepatitis a virus type 1 mediates cell cycle arrest in the s phase
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235186/
https://www.ncbi.nlm.nih.gov/pubmed/35761382
http://dx.doi.org/10.1186/s12985-022-01839-6
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