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Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand

BACKGROUND: Control and elimination of the liver fluke (Opisthorchis viverrini) is a primary preventive strategy against cholangiocarcinoma in Southeast Asia. A sensitive parasitological diagnostic method is required to facilitate a surveillance and control program. In this study, we evaluated the p...

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Autores principales: Kopolrat, Kulthida Y., Singthong, Seri, Khuntikeo, Narong, Loilome, Watcharin, Worasith, Chanika, Homwong, Chutima, Wangboon, Chompunoot, Yasaka, Patiwat, Eamudomkarn, Chatanun, Pitaksakulrat, Opal, Tonkhamhak, Krisnakorn, Paeyo, Arunee, Crellen, Thomas, Sithithaworn, Jiraporn, Sithithaworn, Paiboon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235228/
https://www.ncbi.nlm.nih.gov/pubmed/35761311
http://dx.doi.org/10.1186/s13071-022-05338-z
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author Kopolrat, Kulthida Y.
Singthong, Seri
Khuntikeo, Narong
Loilome, Watcharin
Worasith, Chanika
Homwong, Chutima
Wangboon, Chompunoot
Yasaka, Patiwat
Eamudomkarn, Chatanun
Pitaksakulrat, Opal
Tonkhamhak, Krisnakorn
Paeyo, Arunee
Crellen, Thomas
Sithithaworn, Jiraporn
Sithithaworn, Paiboon
author_facet Kopolrat, Kulthida Y.
Singthong, Seri
Khuntikeo, Narong
Loilome, Watcharin
Worasith, Chanika
Homwong, Chutima
Wangboon, Chompunoot
Yasaka, Patiwat
Eamudomkarn, Chatanun
Pitaksakulrat, Opal
Tonkhamhak, Krisnakorn
Paeyo, Arunee
Crellen, Thomas
Sithithaworn, Jiraporn
Sithithaworn, Paiboon
author_sort Kopolrat, Kulthida Y.
collection PubMed
description BACKGROUND: Control and elimination of the liver fluke (Opisthorchis viverrini) is a primary preventive strategy against cholangiocarcinoma in Southeast Asia. A sensitive parasitological diagnostic method is required to facilitate a surveillance and control program. In this study, we evaluated the performance of Mini Parasep(®) SF stool concentrator kit (stool kit) compared with Kato-Katz (KK) and the quantitative formalin-ethyl acetate concentration technique (FECT) for detection of O. viverrini and co-endemic parasitic infections. METHODS: A cross-sectional survey for parasitic infection in residents aged > 15 years in a community in Kalasin province, Northeast Thailand, was conducted in 2018. Fecal samples were collected and screened by KK method, and a subset of samples was further examined by the stool kit and FECT methods. The results were analyzed for prevalence of parasitic infections in addition to the diagnostic performance of the methods for qualitative and quantitative detection of helminthiases. RESULTS: The initial survey of parasitic infection determined by the KK method (n = 567) showed the prevalence of O. viverrini was 32.63%, followed by Taenia 2.65%, echinostomes 1.76%, hookworms 1.41%, Trichuris trichiura 0.53% and Strongyloides stercoralis 0.53%. Within a subset of samples tested with multiple diagnostics (n = 150), the detection rates of O. viverrini by the stool kit, FECT and KK methods were 27.3%, 30.7% and 28.7%, respectively. The diagnostic sensitivity for opisthorchiasis was similar for FECT (75.5%), KK(66.0%) and the stool kit (67.3%). For other parasitic infections, FECT and stool kit methods performed better than KK, particularly in detecting minute intestinal flukes (MIF), S. stercoralis and coinfections. When measuring the intensity of O. viverrini infection (fecal egg counts), the stool kit results showed a significant positive correlation with KK and FECT (P < 0.05). CONCLUSIONS: As the stool kit is simple to use and shows a comparable performance to FECT, it may serve as an alternative method of fecal examination for screening of helminthiasis including opisthorchiasis. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05338-z.
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spelling pubmed-92352282022-06-28 Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand Kopolrat, Kulthida Y. Singthong, Seri Khuntikeo, Narong Loilome, Watcharin Worasith, Chanika Homwong, Chutima Wangboon, Chompunoot Yasaka, Patiwat Eamudomkarn, Chatanun Pitaksakulrat, Opal Tonkhamhak, Krisnakorn Paeyo, Arunee Crellen, Thomas Sithithaworn, Jiraporn Sithithaworn, Paiboon Parasit Vectors Research BACKGROUND: Control and elimination of the liver fluke (Opisthorchis viverrini) is a primary preventive strategy against cholangiocarcinoma in Southeast Asia. A sensitive parasitological diagnostic method is required to facilitate a surveillance and control program. In this study, we evaluated the performance of Mini Parasep(®) SF stool concentrator kit (stool kit) compared with Kato-Katz (KK) and the quantitative formalin-ethyl acetate concentration technique (FECT) for detection of O. viverrini and co-endemic parasitic infections. METHODS: A cross-sectional survey for parasitic infection in residents aged > 15 years in a community in Kalasin province, Northeast Thailand, was conducted in 2018. Fecal samples were collected and screened by KK method, and a subset of samples was further examined by the stool kit and FECT methods. The results were analyzed for prevalence of parasitic infections in addition to the diagnostic performance of the methods for qualitative and quantitative detection of helminthiases. RESULTS: The initial survey of parasitic infection determined by the KK method (n = 567) showed the prevalence of O. viverrini was 32.63%, followed by Taenia 2.65%, echinostomes 1.76%, hookworms 1.41%, Trichuris trichiura 0.53% and Strongyloides stercoralis 0.53%. Within a subset of samples tested with multiple diagnostics (n = 150), the detection rates of O. viverrini by the stool kit, FECT and KK methods were 27.3%, 30.7% and 28.7%, respectively. The diagnostic sensitivity for opisthorchiasis was similar for FECT (75.5%), KK(66.0%) and the stool kit (67.3%). For other parasitic infections, FECT and stool kit methods performed better than KK, particularly in detecting minute intestinal flukes (MIF), S. stercoralis and coinfections. When measuring the intensity of O. viverrini infection (fecal egg counts), the stool kit results showed a significant positive correlation with KK and FECT (P < 0.05). CONCLUSIONS: As the stool kit is simple to use and shows a comparable performance to FECT, it may serve as an alternative method of fecal examination for screening of helminthiasis including opisthorchiasis. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13071-022-05338-z. BioMed Central 2022-06-27 /pmc/articles/PMC9235228/ /pubmed/35761311 http://dx.doi.org/10.1186/s13071-022-05338-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kopolrat, Kulthida Y.
Singthong, Seri
Khuntikeo, Narong
Loilome, Watcharin
Worasith, Chanika
Homwong, Chutima
Wangboon, Chompunoot
Yasaka, Patiwat
Eamudomkarn, Chatanun
Pitaksakulrat, Opal
Tonkhamhak, Krisnakorn
Paeyo, Arunee
Crellen, Thomas
Sithithaworn, Jiraporn
Sithithaworn, Paiboon
Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand
title Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand
title_full Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand
title_fullStr Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand
title_full_unstemmed Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand
title_short Performance of Mini Parasep(®) SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand
title_sort performance of mini parasep(®) sf stool concentrator kit, kato-katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in northeast thailand
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235228/
https://www.ncbi.nlm.nih.gov/pubmed/35761311
http://dx.doi.org/10.1186/s13071-022-05338-z
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