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An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits

OBJECTIVES: Lead exposure has destructive effects on some organs. It may produce a variety of toxic effects on endothelial cells of the vascular system. Any changes or damages to endothelial cells may lead to cardiovascular diseases, particularly the formation of atherosclerotic plaques. The aim of...

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Autores principales: Vahedi, Parviz, Rajabzadeh, Asghar, Soleimani, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235302/
https://www.ncbi.nlm.nih.gov/pubmed/35769490
http://dx.doi.org/10.1177/20503121221105330
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author Vahedi, Parviz
Rajabzadeh, Asghar
Soleimani, Ali
author_facet Vahedi, Parviz
Rajabzadeh, Asghar
Soleimani, Ali
author_sort Vahedi, Parviz
collection PubMed
description OBJECTIVES: Lead exposure has destructive effects on some organs. It may produce a variety of toxic effects on endothelial cells of the vascular system. Any changes or damages to endothelial cells may lead to cardiovascular diseases, particularly the formation of atherosclerotic plaques. The aim of this study was to determine the ameliorative effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-exposed rabbits. METHODS: In this study, 30 white male rabbits of New Zealand race (weighing about 1.6–2 kg and 5 months old) were used and divided randomly into three groups: Group 1 (N = 10) that served as the control and received water and normal diet, Group 2 (N = 10) was exposed to lead acetate 547 ppm (5 mg/L) daily for 40 days, and Group 3 (N = 10) received vitamin C (500 mg/kg) and underwent the same duration of lead exposure (5 mg/L) daily for 40 days. The levels of cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein were measured using spectrophotometry, and the level of blood lead was calculated using a lead analyzer (Magellan Diagnostics, USA). The animals were anesthetized by pentobarbital (50 mg/kg). Subsequently, they were sacrificed, and their thoracic aortas and coronary arteries were removed. Then fixation, tissue processing, histological sectioning, and H & E staining were carried out. Finally, the sections were studied using light microscopy. The results were analyzed using the Mann–Whitney test. RESULTS: The results indicated that ascorbic acid could reduce the destructive effects of lead on vascular endothelial cells and prevent the formation of atherosclerotic plaques in coronary and aorta arteries. CONCLUSION: The results of this study confirm the beneficial effects of ascorbic acid against the destructive effects of lead on vascular endothelial cells. Hence, it could be proposed as a potential prophylactic treatment for the amelioration of lead toxicity, prevention of atherosclerosis, and improvement of endothelial cells dysfunction.
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spelling pubmed-92353022022-06-28 An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits Vahedi, Parviz Rajabzadeh, Asghar Soleimani, Ali SAGE Open Med Original Research Article OBJECTIVES: Lead exposure has destructive effects on some organs. It may produce a variety of toxic effects on endothelial cells of the vascular system. Any changes or damages to endothelial cells may lead to cardiovascular diseases, particularly the formation of atherosclerotic plaques. The aim of this study was to determine the ameliorative effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-exposed rabbits. METHODS: In this study, 30 white male rabbits of New Zealand race (weighing about 1.6–2 kg and 5 months old) were used and divided randomly into three groups: Group 1 (N = 10) that served as the control and received water and normal diet, Group 2 (N = 10) was exposed to lead acetate 547 ppm (5 mg/L) daily for 40 days, and Group 3 (N = 10) received vitamin C (500 mg/kg) and underwent the same duration of lead exposure (5 mg/L) daily for 40 days. The levels of cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein were measured using spectrophotometry, and the level of blood lead was calculated using a lead analyzer (Magellan Diagnostics, USA). The animals were anesthetized by pentobarbital (50 mg/kg). Subsequently, they were sacrificed, and their thoracic aortas and coronary arteries were removed. Then fixation, tissue processing, histological sectioning, and H & E staining were carried out. Finally, the sections were studied using light microscopy. The results were analyzed using the Mann–Whitney test. RESULTS: The results indicated that ascorbic acid could reduce the destructive effects of lead on vascular endothelial cells and prevent the formation of atherosclerotic plaques in coronary and aorta arteries. CONCLUSION: The results of this study confirm the beneficial effects of ascorbic acid against the destructive effects of lead on vascular endothelial cells. Hence, it could be proposed as a potential prophylactic treatment for the amelioration of lead toxicity, prevention of atherosclerosis, and improvement of endothelial cells dysfunction. SAGE Publications 2022-06-24 /pmc/articles/PMC9235302/ /pubmed/35769490 http://dx.doi.org/10.1177/20503121221105330 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research Article
Vahedi, Parviz
Rajabzadeh, Asghar
Soleimani, Ali
An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
title An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
title_full An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
title_fullStr An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
title_full_unstemmed An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
title_short An evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
title_sort evaluation of the effects of ascorbic acid on the endothelium of coronary and aorta arteries in lead-intoxicated rabbits
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235302/
https://www.ncbi.nlm.nih.gov/pubmed/35769490
http://dx.doi.org/10.1177/20503121221105330
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