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Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes

The mistletoe Psittacanthus schiedeanus, a keystone species in interaction networks between plants, pollinators, and seed dispersers, infects a wide range of native and non-native tree species of commercial interest. Here, using RNA-seq methodology we assembled the whole circularized quadripartite s...

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Autores principales: Ibarra-Laclette, Enrique, Venancio-Rodríguez, Carlos Ariel, Vásquez-Aguilar, Antonio Acini, Alonso-Sánchez, Alexandro G., Pérez-Torres, Claudia-Anahí, Villafán, Emanuel, Ramírez-Barahona, Santiago, Galicia, Sonia, Sosa, Victoria, Rebollar, Eria A., Lara, Carlos, González-Rodríguez, Antonio, Díaz-Fleisher, Francisco, Ornelas, Juan Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235361/
https://www.ncbi.nlm.nih.gov/pubmed/35769994
http://dx.doi.org/10.3389/fgene.2022.929490
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author Ibarra-Laclette, Enrique
Venancio-Rodríguez, Carlos Ariel
Vásquez-Aguilar, Antonio Acini
Alonso-Sánchez, Alexandro G.
Pérez-Torres, Claudia-Anahí
Villafán, Emanuel
Ramírez-Barahona, Santiago
Galicia, Sonia
Sosa, Victoria
Rebollar, Eria A.
Lara, Carlos
González-Rodríguez, Antonio
Díaz-Fleisher, Francisco
Ornelas, Juan Francisco
author_facet Ibarra-Laclette, Enrique
Venancio-Rodríguez, Carlos Ariel
Vásquez-Aguilar, Antonio Acini
Alonso-Sánchez, Alexandro G.
Pérez-Torres, Claudia-Anahí
Villafán, Emanuel
Ramírez-Barahona, Santiago
Galicia, Sonia
Sosa, Victoria
Rebollar, Eria A.
Lara, Carlos
González-Rodríguez, Antonio
Díaz-Fleisher, Francisco
Ornelas, Juan Francisco
author_sort Ibarra-Laclette, Enrique
collection PubMed
description The mistletoe Psittacanthus schiedeanus, a keystone species in interaction networks between plants, pollinators, and seed dispersers, infects a wide range of native and non-native tree species of commercial interest. Here, using RNA-seq methodology we assembled the whole circularized quadripartite structure of P. schiedeanus chloroplast genome and described changes in the gene expression of the nuclear genomes across time of experimentally inoculated seeds. Of the 140,467 assembled and annotated uniGenes, 2,000 were identified as differentially expressed (DEGs) and were classified in six distinct clusters according to their expression profiles. DEGs were also classified in enriched functional categories related to synthesis, signaling, homoeostasis, and response to auxin and jasmonic acid. Since many orthologs are involved in lateral or adventitious root formation in other plant species, we propose that in P. schiedeanus (and perhaps in other rootless mistletoe species), these genes participate in haustorium formation by complex regulatory networks here described. Lastly, and according to the structural similarities of P. schiedeanus enzymes with those that are involved in host cell wall degradation in fungi, we suggest that a similar enzymatic arsenal is secreted extracellularly and used by mistletoes species to easily parasitize and break through tissues of the host.
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spelling pubmed-92353612022-06-28 Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes Ibarra-Laclette, Enrique Venancio-Rodríguez, Carlos Ariel Vásquez-Aguilar, Antonio Acini Alonso-Sánchez, Alexandro G. Pérez-Torres, Claudia-Anahí Villafán, Emanuel Ramírez-Barahona, Santiago Galicia, Sonia Sosa, Victoria Rebollar, Eria A. Lara, Carlos González-Rodríguez, Antonio Díaz-Fleisher, Francisco Ornelas, Juan Francisco Front Genet Genetics The mistletoe Psittacanthus schiedeanus, a keystone species in interaction networks between plants, pollinators, and seed dispersers, infects a wide range of native and non-native tree species of commercial interest. Here, using RNA-seq methodology we assembled the whole circularized quadripartite structure of P. schiedeanus chloroplast genome and described changes in the gene expression of the nuclear genomes across time of experimentally inoculated seeds. Of the 140,467 assembled and annotated uniGenes, 2,000 were identified as differentially expressed (DEGs) and were classified in six distinct clusters according to their expression profiles. DEGs were also classified in enriched functional categories related to synthesis, signaling, homoeostasis, and response to auxin and jasmonic acid. Since many orthologs are involved in lateral or adventitious root formation in other plant species, we propose that in P. schiedeanus (and perhaps in other rootless mistletoe species), these genes participate in haustorium formation by complex regulatory networks here described. Lastly, and according to the structural similarities of P. schiedeanus enzymes with those that are involved in host cell wall degradation in fungi, we suggest that a similar enzymatic arsenal is secreted extracellularly and used by mistletoes species to easily parasitize and break through tissues of the host. Frontiers Media S.A. 2022-06-13 /pmc/articles/PMC9235361/ /pubmed/35769994 http://dx.doi.org/10.3389/fgene.2022.929490 Text en Copyright © 2022 Ibarra-Laclette, Venancio-Rodríguez, Vásquez-Aguilar, Alonso-Sánchez, Pérez-Torres, Villafán, Ramírez-Barahona, Galicia, Sosa, Rebollar, Lara, González-Rodríguez, Díaz-Fleisher and Ornelas. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ibarra-Laclette, Enrique
Venancio-Rodríguez, Carlos Ariel
Vásquez-Aguilar, Antonio Acini
Alonso-Sánchez, Alexandro G.
Pérez-Torres, Claudia-Anahí
Villafán, Emanuel
Ramírez-Barahona, Santiago
Galicia, Sonia
Sosa, Victoria
Rebollar, Eria A.
Lara, Carlos
González-Rodríguez, Antonio
Díaz-Fleisher, Francisco
Ornelas, Juan Francisco
Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
title Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
title_full Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
title_fullStr Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
title_full_unstemmed Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
title_short Transcriptional Basis for Haustorium Formation and Host Establishment in Hemiparasitic Psittacanthus schiedeanus Mistletoes
title_sort transcriptional basis for haustorium formation and host establishment in hemiparasitic psittacanthus schiedeanus mistletoes
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235361/
https://www.ncbi.nlm.nih.gov/pubmed/35769994
http://dx.doi.org/10.3389/fgene.2022.929490
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