Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells

The widespread two-component system (TCS), QseBC, involves vital virulence regulators in Enterobacteriaceae and Pasteurellaceae. Here we studied the function of QseBC in Glaesserella parasuis. A ΔqseBC mutant was constructed using a Glaesserella parasuis serovar 11 clinical strain SC1401 by natural...

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Autores principales: Yan, Xuefeng, Dai, Ke, Gu, Congwei, Yu, Zehui, He, Manli, Xiao, Wudian, Zhao, Mingde, He, Lvqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235811/
https://www.ncbi.nlm.nih.gov/pubmed/35769141
http://dx.doi.org/10.7717/peerj.13648
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author Yan, Xuefeng
Dai, Ke
Gu, Congwei
Yu, Zehui
He, Manli
Xiao, Wudian
Zhao, Mingde
He, Lvqin
author_facet Yan, Xuefeng
Dai, Ke
Gu, Congwei
Yu, Zehui
He, Manli
Xiao, Wudian
Zhao, Mingde
He, Lvqin
author_sort Yan, Xuefeng
collection PubMed
description The widespread two-component system (TCS), QseBC, involves vital virulence regulators in Enterobacteriaceae and Pasteurellaceae. Here we studied the function of QseBC in Glaesserella parasuis. A ΔqseBC mutant was constructed using a Glaesserella parasuis serovar 11 clinical strain SC1401 by natural transformation. Immunofluorescence was used to evaluate cellular adhesion, the levels of inflammation and apoptosis. The ability of ΔqseBC and ΔqseC mutant strains to adhere to PAM and MLE-12 cells was significantly reduced. Additionally, by focusing on the clinical signs, H&E, and IFA for inflammation and apoptosis, we found that the ΔqseBC mutant weakened virulence in the murine models. Together, these findings suggest that QseBC plays an important role in the virulence of Glaesserella parasuis.
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spelling pubmed-92358112022-06-28 Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells Yan, Xuefeng Dai, Ke Gu, Congwei Yu, Zehui He, Manli Xiao, Wudian Zhao, Mingde He, Lvqin PeerJ Biochemistry The widespread two-component system (TCS), QseBC, involves vital virulence regulators in Enterobacteriaceae and Pasteurellaceae. Here we studied the function of QseBC in Glaesserella parasuis. A ΔqseBC mutant was constructed using a Glaesserella parasuis serovar 11 clinical strain SC1401 by natural transformation. Immunofluorescence was used to evaluate cellular adhesion, the levels of inflammation and apoptosis. The ability of ΔqseBC and ΔqseC mutant strains to adhere to PAM and MLE-12 cells was significantly reduced. Additionally, by focusing on the clinical signs, H&E, and IFA for inflammation and apoptosis, we found that the ΔqseBC mutant weakened virulence in the murine models. Together, these findings suggest that QseBC plays an important role in the virulence of Glaesserella parasuis. PeerJ Inc. 2022-06-24 /pmc/articles/PMC9235811/ /pubmed/35769141 http://dx.doi.org/10.7717/peerj.13648 Text en ©2022 Yan et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Yan, Xuefeng
Dai, Ke
Gu, Congwei
Yu, Zehui
He, Manli
Xiao, Wudian
Zhao, Mingde
He, Lvqin
Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells
title Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells
title_full Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells
title_fullStr Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells
title_full_unstemmed Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells
title_short Deletion of two-component system QseBC weakened virulence of Glaesserella parasuis in a murine acute infection model and adhesion to host cells
title_sort deletion of two-component system qsebc weakened virulence of glaesserella parasuis in a murine acute infection model and adhesion to host cells
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9235811/
https://www.ncbi.nlm.nih.gov/pubmed/35769141
http://dx.doi.org/10.7717/peerj.13648
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