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Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice

Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered...

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Autores principales: Medina-Ruiz, Laura, Bartolini, Robin, Wilson, Gillian J, Dyer, Douglas P, Vidler, Francesca, Hughes, Catherine E, Schuette, Fabian, Love, Samantha, Pingen, Marieke, Hayes, Alan James, Fu, Jun, Stewart, Adrian Francis, Graham, Gerard J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9236609/
https://www.ncbi.nlm.nih.gov/pubmed/35699420
http://dx.doi.org/10.7554/eLife.72418
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author Medina-Ruiz, Laura
Bartolini, Robin
Wilson, Gillian J
Dyer, Douglas P
Vidler, Francesca
Hughes, Catherine E
Schuette, Fabian
Love, Samantha
Pingen, Marieke
Hayes, Alan James
Fu, Jun
Stewart, Adrian Francis
Graham, Gerard J
author_facet Medina-Ruiz, Laura
Bartolini, Robin
Wilson, Gillian J
Dyer, Douglas P
Vidler, Francesca
Hughes, Catherine E
Schuette, Fabian
Love, Samantha
Pingen, Marieke
Hayes, Alan James
Fu, Jun
Stewart, Adrian Francis
Graham, Gerard J
author_sort Medina-Ruiz, Laura
collection PubMed
description Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered attempts at targeting this system in inflammatory disease. Novel approaches are therefore needed to provide crucial biological, and therapeutic, insights into the chemokine-chemokine receptor family. Here, we report the generation of transgenic multi-chemokine receptor reporter mice in which spectrally distinct fluorescent reporters mark expression of CCRs 1, 2, 3, and 5, key receptors for myeloid cell recruitment in inflammation. Analysis of these animals has allowed us to define, for the first time, individual and combinatorial receptor expression patterns on myeloid cells in resting and inflamed conditions. Our results demonstrate that chemokine receptor expression is highly specific, and more selective than previously anticipated.
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spelling pubmed-92366092022-06-28 Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice Medina-Ruiz, Laura Bartolini, Robin Wilson, Gillian J Dyer, Douglas P Vidler, Francesca Hughes, Catherine E Schuette, Fabian Love, Samantha Pingen, Marieke Hayes, Alan James Fu, Jun Stewart, Adrian Francis Graham, Gerard J eLife Immunology and Inflammation Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered attempts at targeting this system in inflammatory disease. Novel approaches are therefore needed to provide crucial biological, and therapeutic, insights into the chemokine-chemokine receptor family. Here, we report the generation of transgenic multi-chemokine receptor reporter mice in which spectrally distinct fluorescent reporters mark expression of CCRs 1, 2, 3, and 5, key receptors for myeloid cell recruitment in inflammation. Analysis of these animals has allowed us to define, for the first time, individual and combinatorial receptor expression patterns on myeloid cells in resting and inflamed conditions. Our results demonstrate that chemokine receptor expression is highly specific, and more selective than previously anticipated. eLife Sciences Publications, Ltd 2022-06-14 /pmc/articles/PMC9236609/ /pubmed/35699420 http://dx.doi.org/10.7554/eLife.72418 Text en © 2022, Medina-Ruiz et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Immunology and Inflammation
Medina-Ruiz, Laura
Bartolini, Robin
Wilson, Gillian J
Dyer, Douglas P
Vidler, Francesca
Hughes, Catherine E
Schuette, Fabian
Love, Samantha
Pingen, Marieke
Hayes, Alan James
Fu, Jun
Stewart, Adrian Francis
Graham, Gerard J
Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
title Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
title_full Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
title_fullStr Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
title_full_unstemmed Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
title_short Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
title_sort analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
topic Immunology and Inflammation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9236609/
https://www.ncbi.nlm.nih.gov/pubmed/35699420
http://dx.doi.org/10.7554/eLife.72418
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