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Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice
Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9236609/ https://www.ncbi.nlm.nih.gov/pubmed/35699420 http://dx.doi.org/10.7554/eLife.72418 |
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author | Medina-Ruiz, Laura Bartolini, Robin Wilson, Gillian J Dyer, Douglas P Vidler, Francesca Hughes, Catherine E Schuette, Fabian Love, Samantha Pingen, Marieke Hayes, Alan James Fu, Jun Stewart, Adrian Francis Graham, Gerard J |
author_facet | Medina-Ruiz, Laura Bartolini, Robin Wilson, Gillian J Dyer, Douglas P Vidler, Francesca Hughes, Catherine E Schuette, Fabian Love, Samantha Pingen, Marieke Hayes, Alan James Fu, Jun Stewart, Adrian Francis Graham, Gerard J |
author_sort | Medina-Ruiz, Laura |
collection | PubMed |
description | Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered attempts at targeting this system in inflammatory disease. Novel approaches are therefore needed to provide crucial biological, and therapeutic, insights into the chemokine-chemokine receptor family. Here, we report the generation of transgenic multi-chemokine receptor reporter mice in which spectrally distinct fluorescent reporters mark expression of CCRs 1, 2, 3, and 5, key receptors for myeloid cell recruitment in inflammation. Analysis of these animals has allowed us to define, for the first time, individual and combinatorial receptor expression patterns on myeloid cells in resting and inflamed conditions. Our results demonstrate that chemokine receptor expression is highly specific, and more selective than previously anticipated. |
format | Online Article Text |
id | pubmed-9236609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-92366092022-06-28 Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice Medina-Ruiz, Laura Bartolini, Robin Wilson, Gillian J Dyer, Douglas P Vidler, Francesca Hughes, Catherine E Schuette, Fabian Love, Samantha Pingen, Marieke Hayes, Alan James Fu, Jun Stewart, Adrian Francis Graham, Gerard J eLife Immunology and Inflammation Inflammatory chemokines and their receptors are central to the development of inflammatory/immune pathologies. The apparent complexity of this system, coupled with lack of appropriate in vivo models, has limited our understanding of how chemokines orchestrate inflammatory responses and has hampered attempts at targeting this system in inflammatory disease. Novel approaches are therefore needed to provide crucial biological, and therapeutic, insights into the chemokine-chemokine receptor family. Here, we report the generation of transgenic multi-chemokine receptor reporter mice in which spectrally distinct fluorescent reporters mark expression of CCRs 1, 2, 3, and 5, key receptors for myeloid cell recruitment in inflammation. Analysis of these animals has allowed us to define, for the first time, individual and combinatorial receptor expression patterns on myeloid cells in resting and inflamed conditions. Our results demonstrate that chemokine receptor expression is highly specific, and more selective than previously anticipated. eLife Sciences Publications, Ltd 2022-06-14 /pmc/articles/PMC9236609/ /pubmed/35699420 http://dx.doi.org/10.7554/eLife.72418 Text en © 2022, Medina-Ruiz et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Immunology and Inflammation Medina-Ruiz, Laura Bartolini, Robin Wilson, Gillian J Dyer, Douglas P Vidler, Francesca Hughes, Catherine E Schuette, Fabian Love, Samantha Pingen, Marieke Hayes, Alan James Fu, Jun Stewart, Adrian Francis Graham, Gerard J Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
title | Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
title_full | Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
title_fullStr | Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
title_full_unstemmed | Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
title_short | Analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
title_sort | analysis of combinatorial chemokine receptor expression dynamics using multi-receptor reporter mice |
topic | Immunology and Inflammation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9236609/ https://www.ncbi.nlm.nih.gov/pubmed/35699420 http://dx.doi.org/10.7554/eLife.72418 |
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