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Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study

Early flowering, maturity, and plant height are important traits for linseed to fit in rice fallows, for rainfed agriculture, and for economically viable cultivation. Here, Multi-Locus Genome-Wide Association Study (ML-GWAS) was undertaken in an association mapping panel of 131 accessions, genotyped...

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Autores principales: Saroha, Ankit, Pal, Deepa, Gomashe, Sunil S., Akash, Kaur, Vikender, Ujjainwal, Shraddha, Rajkumar, S., Aravind, J., Radhamani, J., Kumar, Rajesh, Chand, Dinesh, Sengupta, Abhishek, Wankhede, Dhammaprakash Pandhari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237403/
https://www.ncbi.nlm.nih.gov/pubmed/35774513
http://dx.doi.org/10.3389/fgene.2022.811924
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author Saroha, Ankit
Pal, Deepa
Gomashe, Sunil S.
Akash,
Kaur, Vikender
Ujjainwal, Shraddha
Rajkumar, S.
Aravind, J.
Radhamani, J.
Kumar, Rajesh
Chand, Dinesh
Sengupta, Abhishek
Wankhede, Dhammaprakash Pandhari
author_facet Saroha, Ankit
Pal, Deepa
Gomashe, Sunil S.
Akash,
Kaur, Vikender
Ujjainwal, Shraddha
Rajkumar, S.
Aravind, J.
Radhamani, J.
Kumar, Rajesh
Chand, Dinesh
Sengupta, Abhishek
Wankhede, Dhammaprakash Pandhari
author_sort Saroha, Ankit
collection PubMed
description Early flowering, maturity, and plant height are important traits for linseed to fit in rice fallows, for rainfed agriculture, and for economically viable cultivation. Here, Multi-Locus Genome-Wide Association Study (ML-GWAS) was undertaken in an association mapping panel of 131 accessions, genotyped using 68,925 SNPs identified by genotyping by sequencing approach. Phenotypic evaluation data of five environments comprising 3 years and two locations were used. GWAS was performed for three flowering time traits including days to 5%, 50%, and 95% flowering, days to maturity, and plant height by employing five ML-GWAS methods: FASTmrEMMA, FASTmrMLM, ISIS EM-BLASSO, mrMLM, and pLARmEB. A total of 335 unique QTNs have been identified for five traits across five environments. 109 QTNs were stable as observed in ≥2 methods and/or environments, explaining up to 36.6% phenotypic variance. For three flowering time traits, days to maturity, and plant height, 53, 30, and 27 stable QTNs, respectively, were identified. Candidate genes having roles in flower, pollen, embryo, seed and fruit development, and xylem/phloem histogenesis have been identified. Gene expression of candidate genes for flowering and plant height were studied using transcriptome of an early maturing variety Sharda (IC0523807). The present study unravels QTNs/candidate genes underlying complex flowering, days to maturity, and plant height traits in linseed.
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spelling pubmed-92374032022-06-29 Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study Saroha, Ankit Pal, Deepa Gomashe, Sunil S. Akash, Kaur, Vikender Ujjainwal, Shraddha Rajkumar, S. Aravind, J. Radhamani, J. Kumar, Rajesh Chand, Dinesh Sengupta, Abhishek Wankhede, Dhammaprakash Pandhari Front Genet Genetics Early flowering, maturity, and plant height are important traits for linseed to fit in rice fallows, for rainfed agriculture, and for economically viable cultivation. Here, Multi-Locus Genome-Wide Association Study (ML-GWAS) was undertaken in an association mapping panel of 131 accessions, genotyped using 68,925 SNPs identified by genotyping by sequencing approach. Phenotypic evaluation data of five environments comprising 3 years and two locations were used. GWAS was performed for three flowering time traits including days to 5%, 50%, and 95% flowering, days to maturity, and plant height by employing five ML-GWAS methods: FASTmrEMMA, FASTmrMLM, ISIS EM-BLASSO, mrMLM, and pLARmEB. A total of 335 unique QTNs have been identified for five traits across five environments. 109 QTNs were stable as observed in ≥2 methods and/or environments, explaining up to 36.6% phenotypic variance. For three flowering time traits, days to maturity, and plant height, 53, 30, and 27 stable QTNs, respectively, were identified. Candidate genes having roles in flower, pollen, embryo, seed and fruit development, and xylem/phloem histogenesis have been identified. Gene expression of candidate genes for flowering and plant height were studied using transcriptome of an early maturing variety Sharda (IC0523807). The present study unravels QTNs/candidate genes underlying complex flowering, days to maturity, and plant height traits in linseed. Frontiers Media S.A. 2022-06-14 /pmc/articles/PMC9237403/ /pubmed/35774513 http://dx.doi.org/10.3389/fgene.2022.811924 Text en Copyright © 2022 Saroha, Pal, Gomashe, Akash, Kaur, Ujjainwal, Rajkumar, Aravind, Radhamani, Kumar, Chand, Sengupta and Wankhede. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Saroha, Ankit
Pal, Deepa
Gomashe, Sunil S.
Akash,
Kaur, Vikender
Ujjainwal, Shraddha
Rajkumar, S.
Aravind, J.
Radhamani, J.
Kumar, Rajesh
Chand, Dinesh
Sengupta, Abhishek
Wankhede, Dhammaprakash Pandhari
Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study
title Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study
title_full Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study
title_fullStr Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study
title_full_unstemmed Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study
title_short Identification of QTNs Associated With Flowering Time, Maturity, and Plant Height Traits in Linum usitatissimum L. Using Genome-Wide Association Study
title_sort identification of qtns associated with flowering time, maturity, and plant height traits in linum usitatissimum l. using genome-wide association study
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237403/
https://www.ncbi.nlm.nih.gov/pubmed/35774513
http://dx.doi.org/10.3389/fgene.2022.811924
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