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Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differen...

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Autores principales: Gao, Yan, Tian, Zeyu, Liu, Qian, Wang, Ting, Ban, Lee-Kiat, Lee, Henry Hsin-Chung, Umezawa, Akihiro, Almansour, Abdulrahman I., Arumugam, Natarajan, Kumar, Raju Suresh, Ye, Qingsong, Higuchi, Akon, Chen, Hao, Sung, Tzu-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237518/
https://www.ncbi.nlm.nih.gov/pubmed/35774224
http://dx.doi.org/10.3389/fcell.2022.893241
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author Gao, Yan
Tian, Zeyu
Liu, Qian
Wang, Ting
Ban, Lee-Kiat
Lee, Henry Hsin-Chung
Umezawa, Akihiro
Almansour, Abdulrahman I.
Arumugam, Natarajan
Kumar, Raju Suresh
Ye, Qingsong
Higuchi, Akon
Chen, Hao
Sung, Tzu-Cheng
author_facet Gao, Yan
Tian, Zeyu
Liu, Qian
Wang, Ting
Ban, Lee-Kiat
Lee, Henry Hsin-Chung
Umezawa, Akihiro
Almansour, Abdulrahman I.
Arumugam, Natarajan
Kumar, Raju Suresh
Ye, Qingsong
Higuchi, Akon
Chen, Hao
Sung, Tzu-Cheng
author_sort Gao, Yan
collection PubMed
description Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future.
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spelling pubmed-92375182022-06-29 Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins Gao, Yan Tian, Zeyu Liu, Qian Wang, Ting Ban, Lee-Kiat Lee, Henry Hsin-Chung Umezawa, Akihiro Almansour, Abdulrahman I. Arumugam, Natarajan Kumar, Raju Suresh Ye, Qingsong Higuchi, Akon Chen, Hao Sung, Tzu-Cheng Front Cell Dev Biol Cell and Developmental Biology Stem cells serve as an ideal source of tissue regeneration therapy because of their high stemness properties and regenerative activities. Mesenchymal stem cells (MSCs) are considered an excellent source of stem cell therapy because MSCs can be easily obtained without ethical concern and can differentiate into most types of cells in the human body. We prepared cell culture materials combined with synthetic polymeric materials of poly-N-isopropylacrylamide-co-butyl acrylate (PN) and extracellular matrix proteins to investigate the effect of cell culture biomaterials on the differentiation of dental pulp stem cells (DPSCs) into neuronal cells. The DPSCs cultured on poly-L-ornithine (PLO)-coated (TPS-PLO) plates and PLO and PN-coated (TPS-PLO-PN) plates showed excellent neuronal marker (βIII-tubulin and nestin) expression and the highest expansion rate among the culture plates investigated in this study. This result suggests that the TPS-PLO and TPS-PN-PLO plates maintained stable DPSCs proliferation and had good capabilities of differentiating into neuronal cells. TPS-PLO and TPS-PN-PLO plates may have high potentials as cell culture biomaterials for the differentiation of MSCs into several neural cells, such as cells in the central nervous system, retinal cells, retinal organoids and oligodendrocytes, which will expand the sources of cells for stem cell therapies in the future. Frontiers Media S.A. 2022-06-14 /pmc/articles/PMC9237518/ /pubmed/35774224 http://dx.doi.org/10.3389/fcell.2022.893241 Text en Copyright © 2022 Gao, Tian, Liu, Wang, Ban, Lee, Umezawa, Almansour, Arumugam, Kumar, Ye, Higuchi, Chen and Sung. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Gao, Yan
Tian, Zeyu
Liu, Qian
Wang, Ting
Ban, Lee-Kiat
Lee, Henry Hsin-Chung
Umezawa, Akihiro
Almansour, Abdulrahman I.
Arumugam, Natarajan
Kumar, Raju Suresh
Ye, Qingsong
Higuchi, Akon
Chen, Hao
Sung, Tzu-Cheng
Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins
title Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins
title_full Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins
title_fullStr Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins
title_full_unstemmed Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins
title_short Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins
title_sort neuronal cell differentiation of human dental pulp stem cells on synthetic polymeric surfaces coated with ecm proteins
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237518/
https://www.ncbi.nlm.nih.gov/pubmed/35774224
http://dx.doi.org/10.3389/fcell.2022.893241
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