Cargando…
In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control
Obesity-related diseases are always the major health problems that concern the whole world. Serial studies have reported that obesity development is closely related to the out-of-control leptin encoded by the obesity gene (ob). The latest report declaimed “Less Is More,” a model explaining that part...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237534/ https://www.ncbi.nlm.nih.gov/pubmed/35774455 http://dx.doi.org/10.3389/fmicb.2022.923656 |
_version_ | 1784736817422008320 |
---|---|
author | Yue, Feng Du, Lihong Wang, Ruyu Han, Baoquan Zhang, Xiaojun Yao, Zhangzhang Zhang, Wenqiang Cai, Chang Zhang, Zhiying Xu, Kun |
author_facet | Yue, Feng Du, Lihong Wang, Ruyu Han, Baoquan Zhang, Xiaojun Yao, Zhangzhang Zhang, Wenqiang Cai, Chang Zhang, Zhiying Xu, Kun |
author_sort | Yue, Feng |
collection | PubMed |
description | Obesity-related diseases are always the major health problems that concern the whole world. Serial studies have reported that obesity development is closely related to the out-of-control leptin encoded by the obesity gene (ob). The latest report declaimed “Less Is More,” a model explaining that partial leptin reduction triggers leptin sensitization and contributes to obesity control. Here, we came up with a novel concept, in vivo protein interference (iPRTi), an interesting protein knock-down strategy for in vivo partial leptin reduction. First, the specific immune response against leptin induced by the oral administration of ob recombinant yeast was confirmed. Subsequentally, leptin resistance was observed in diet-induced obese mice, and oral administration with ob recombinant yeast declined the circulating leptin and reduced significantly the body weight gain. To further investigate whether the iPRTi strategy is capable of obesity management, the diet-induced obese mice were administrated with ob recombinant yeast. All the indexes examined including the circulating leptin, triglyceride, and total cholesterol, as well as food intake and weight gain, demonstrated a positive effect of the iPRTi strategy on obesity control. In short, this study provides a novel strategy for the potential application of recombinant yeast for the therapy of obese individuals with leptin resistance. |
format | Online Article Text |
id | pubmed-9237534 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92375342022-06-29 In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control Yue, Feng Du, Lihong Wang, Ruyu Han, Baoquan Zhang, Xiaojun Yao, Zhangzhang Zhang, Wenqiang Cai, Chang Zhang, Zhiying Xu, Kun Front Microbiol Microbiology Obesity-related diseases are always the major health problems that concern the whole world. Serial studies have reported that obesity development is closely related to the out-of-control leptin encoded by the obesity gene (ob). The latest report declaimed “Less Is More,” a model explaining that partial leptin reduction triggers leptin sensitization and contributes to obesity control. Here, we came up with a novel concept, in vivo protein interference (iPRTi), an interesting protein knock-down strategy for in vivo partial leptin reduction. First, the specific immune response against leptin induced by the oral administration of ob recombinant yeast was confirmed. Subsequentally, leptin resistance was observed in diet-induced obese mice, and oral administration with ob recombinant yeast declined the circulating leptin and reduced significantly the body weight gain. To further investigate whether the iPRTi strategy is capable of obesity management, the diet-induced obese mice were administrated with ob recombinant yeast. All the indexes examined including the circulating leptin, triglyceride, and total cholesterol, as well as food intake and weight gain, demonstrated a positive effect of the iPRTi strategy on obesity control. In short, this study provides a novel strategy for the potential application of recombinant yeast for the therapy of obese individuals with leptin resistance. Frontiers Media S.A. 2022-06-14 /pmc/articles/PMC9237534/ /pubmed/35774455 http://dx.doi.org/10.3389/fmicb.2022.923656 Text en Copyright © 2022 Yue, Du, Wang, Han, Zhang, Yao, Zhang, Cai, Zhang and Xu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yue, Feng Du, Lihong Wang, Ruyu Han, Baoquan Zhang, Xiaojun Yao, Zhangzhang Zhang, Wenqiang Cai, Chang Zhang, Zhiying Xu, Kun In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control |
title | In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control |
title_full | In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control |
title_fullStr | In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control |
title_full_unstemmed | In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control |
title_short | In vivo Protein Interference: Oral Administration of Recombinant Yeast-Mediated Partial Leptin Reduction for Obesity Control |
title_sort | in vivo protein interference: oral administration of recombinant yeast-mediated partial leptin reduction for obesity control |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237534/ https://www.ncbi.nlm.nih.gov/pubmed/35774455 http://dx.doi.org/10.3389/fmicb.2022.923656 |
work_keys_str_mv | AT yuefeng invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT dulihong invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT wangruyu invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT hanbaoquan invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT zhangxiaojun invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT yaozhangzhang invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT zhangwenqiang invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT caichang invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT zhangzhiying invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol AT xukun invivoproteininterferenceoraladministrationofrecombinantyeastmediatedpartialleptinreductionforobesitycontrol |