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Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms
The detection and co-circulation of multiple variants of porcine reproductive and respiratory syndrome virus (PRRSV) have been observed and reported in swine. However, the potential long-term impact of multiple prevailing PRRSV variants on pig-performance is not yet fully understood. The primary obj...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237545/ https://www.ncbi.nlm.nih.gov/pubmed/35774978 http://dx.doi.org/10.3389/fvets.2022.884733 |
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author | Cheng, Ting-Yu Campler, Magnus R. Schroeder, Declan C. Yang, My Mor, Sunil K. Ferreira, Juliana B. Arruda, Andréia G. |
author_facet | Cheng, Ting-Yu Campler, Magnus R. Schroeder, Declan C. Yang, My Mor, Sunil K. Ferreira, Juliana B. Arruda, Andréia G. |
author_sort | Cheng, Ting-Yu |
collection | PubMed |
description | The detection and co-circulation of multiple variants of porcine reproductive and respiratory syndrome virus (PRRSV) have been observed and reported in swine. However, the potential long-term impact of multiple prevailing PRRSV variants on pig-performance is not yet fully understood. The primary objective of this study was to describe the genetic variation of PRRSV in processing fluid (PF), oral fluid (OF), and tonsil scraping (TS) specimens from five swine farms with different production types and PRRS status over a period of time (~1 year). Furthermore, the association between PRRSV prevalence and production parameters was investigated. Results showed that PRRSV was detected by RT-qPCR in 21–25% of all types of specimens. In breeding farms, PRRSV detection in PF and/or TS samples was correlated with stillborn and mummified fetuses, and pre-weaning mortality throughout the study period. Although ORF5 sequences were obtained in <16% of all sample types, simultaneous detection of PRRSV variants including field and vaccine strains within a single sampling event was identified in both breeding and growing pig farms. Phylogenetic analyses based on the ORF5 sequence classified the detected field PRRSV into L1A and L1H, two sub-lineages of lineage 1 (L1). Our study demonstrated the presence of multiple PRRSV lineages, sub-lineages, and variants in swine herds and its potential association with swine reproductive performance under field conditions. |
format | Online Article Text |
id | pubmed-9237545 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92375452022-06-29 Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms Cheng, Ting-Yu Campler, Magnus R. Schroeder, Declan C. Yang, My Mor, Sunil K. Ferreira, Juliana B. Arruda, Andréia G. Front Vet Sci Veterinary Science The detection and co-circulation of multiple variants of porcine reproductive and respiratory syndrome virus (PRRSV) have been observed and reported in swine. However, the potential long-term impact of multiple prevailing PRRSV variants on pig-performance is not yet fully understood. The primary objective of this study was to describe the genetic variation of PRRSV in processing fluid (PF), oral fluid (OF), and tonsil scraping (TS) specimens from five swine farms with different production types and PRRS status over a period of time (~1 year). Furthermore, the association between PRRSV prevalence and production parameters was investigated. Results showed that PRRSV was detected by RT-qPCR in 21–25% of all types of specimens. In breeding farms, PRRSV detection in PF and/or TS samples was correlated with stillborn and mummified fetuses, and pre-weaning mortality throughout the study period. Although ORF5 sequences were obtained in <16% of all sample types, simultaneous detection of PRRSV variants including field and vaccine strains within a single sampling event was identified in both breeding and growing pig farms. Phylogenetic analyses based on the ORF5 sequence classified the detected field PRRSV into L1A and L1H, two sub-lineages of lineage 1 (L1). Our study demonstrated the presence of multiple PRRSV lineages, sub-lineages, and variants in swine herds and its potential association with swine reproductive performance under field conditions. Frontiers Media S.A. 2022-06-14 /pmc/articles/PMC9237545/ /pubmed/35774978 http://dx.doi.org/10.3389/fvets.2022.884733 Text en Copyright © 2022 Cheng, Campler, Schroeder, Yang, Mor, Ferreira and Arruda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Cheng, Ting-Yu Campler, Magnus R. Schroeder, Declan C. Yang, My Mor, Sunil K. Ferreira, Juliana B. Arruda, Andréia G. Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms |
title | Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms |
title_full | Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms |
title_fullStr | Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms |
title_full_unstemmed | Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms |
title_short | Detection of Multiple Lineages of PRRSV in Breeding and Growing Swine Farms |
title_sort | detection of multiple lineages of prrsv in breeding and growing swine farms |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237545/ https://www.ncbi.nlm.nih.gov/pubmed/35774978 http://dx.doi.org/10.3389/fvets.2022.884733 |
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