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Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways

Cinnamomum zeylanicum is a tropical plant with traditional medicinal significance that possesses antimicrobial, antifungal, anti-parasitic, and anti-tumor properties. Here, we have elucidated the anti-tumor effects of Cinnamomum zeylanicum extract (CZE) and its bioactive compound cinnamaldehyde (CIN...

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Autores principales: Aggarwal, Sadhna, Bhadana, Kanchan, Singh, Baldeep, Rawat, Meenakshi, Mohammad, Taj, Al-Keridis, Lamya Ahmed, Alshammari, Nawaf, Hassan, Md. Imtaiyaz, Das, Satya N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237655/
https://www.ncbi.nlm.nih.gov/pubmed/35774603
http://dx.doi.org/10.3389/fphar.2022.918479
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author Aggarwal, Sadhna
Bhadana, Kanchan
Singh, Baldeep
Rawat, Meenakshi
Mohammad, Taj
Al-Keridis, Lamya Ahmed
Alshammari, Nawaf
Hassan, Md. Imtaiyaz
Das, Satya N.
author_facet Aggarwal, Sadhna
Bhadana, Kanchan
Singh, Baldeep
Rawat, Meenakshi
Mohammad, Taj
Al-Keridis, Lamya Ahmed
Alshammari, Nawaf
Hassan, Md. Imtaiyaz
Das, Satya N.
author_sort Aggarwal, Sadhna
collection PubMed
description Cinnamomum zeylanicum is a tropical plant with traditional medicinal significance that possesses antimicrobial, antifungal, anti-parasitic, and anti-tumor properties. Here, we have elucidated the anti-tumor effects of Cinnamomum zeylanicum extract (CZE) and its bioactive compound cinnamaldehyde (CIN) on oral cancer and elucidated underlying molecular mechanisms. Anti-tumor activities of CZE and CIN were demonstrated by various in vitro experiments on oral cancer cells (SCC-4, SCC-9, SCC-25). The cell proliferation, growth, cell cycle arrest, apoptosis, and autophagy were analyzed by MTT, clonogenic assay, propidium iodide, annexin-V-PI, DAPI, and acridine orange staining, respectively. The binding affinity of CIN towards dihydrofolate reductase and p38-MAP kinase alpha was analyzed by molecular docking. Western blot assay was performed to assess the alteration in the expression of various proteins. CZE and CIN treatment significantly inhibited the growth and proliferation of oral cancer cells in a dose-dependent manner. These treatments further induced apoptosis, cell cycle arrest, and autophagy. CZE and CIN inhibited the invasion and cytoplasmic translocation of NF-κB in these cell lines. CIN showed a high affinity to MAP kinase P38 alpha and dihydrofolate reductase with binding affinities of −6.8 and −5.9 kcal/mol, respectively. The cancer cells showed a decreased expression of various PI3k-AKT-mTOR pathways related to VEGF, COX-2, Bcl-2, NF-κB, and proteins post-treatment.
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spelling pubmed-92376552022-06-29 Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways Aggarwal, Sadhna Bhadana, Kanchan Singh, Baldeep Rawat, Meenakshi Mohammad, Taj Al-Keridis, Lamya Ahmed Alshammari, Nawaf Hassan, Md. Imtaiyaz Das, Satya N. Front Pharmacol Pharmacology Cinnamomum zeylanicum is a tropical plant with traditional medicinal significance that possesses antimicrobial, antifungal, anti-parasitic, and anti-tumor properties. Here, we have elucidated the anti-tumor effects of Cinnamomum zeylanicum extract (CZE) and its bioactive compound cinnamaldehyde (CIN) on oral cancer and elucidated underlying molecular mechanisms. Anti-tumor activities of CZE and CIN were demonstrated by various in vitro experiments on oral cancer cells (SCC-4, SCC-9, SCC-25). The cell proliferation, growth, cell cycle arrest, apoptosis, and autophagy were analyzed by MTT, clonogenic assay, propidium iodide, annexin-V-PI, DAPI, and acridine orange staining, respectively. The binding affinity of CIN towards dihydrofolate reductase and p38-MAP kinase alpha was analyzed by molecular docking. Western blot assay was performed to assess the alteration in the expression of various proteins. CZE and CIN treatment significantly inhibited the growth and proliferation of oral cancer cells in a dose-dependent manner. These treatments further induced apoptosis, cell cycle arrest, and autophagy. CZE and CIN inhibited the invasion and cytoplasmic translocation of NF-κB in these cell lines. CIN showed a high affinity to MAP kinase P38 alpha and dihydrofolate reductase with binding affinities of −6.8 and −5.9 kcal/mol, respectively. The cancer cells showed a decreased expression of various PI3k-AKT-mTOR pathways related to VEGF, COX-2, Bcl-2, NF-κB, and proteins post-treatment. Frontiers Media S.A. 2022-06-02 /pmc/articles/PMC9237655/ /pubmed/35774603 http://dx.doi.org/10.3389/fphar.2022.918479 Text en Copyright © 2022 Aggarwal, Bhadana, Singh, Rawat, Mohammad, Al-Keridis, Alshammari, Hassan and Das. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Aggarwal, Sadhna
Bhadana, Kanchan
Singh, Baldeep
Rawat, Meenakshi
Mohammad, Taj
Al-Keridis, Lamya Ahmed
Alshammari, Nawaf
Hassan, Md. Imtaiyaz
Das, Satya N.
Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways
title Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways
title_full Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways
title_fullStr Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways
title_full_unstemmed Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways
title_short Cinnamomum zeylanicum Extract and its Bioactive Component Cinnamaldehyde Show Anti-Tumor Effects via Inhibition of Multiple Cellular Pathways
title_sort cinnamomum zeylanicum extract and its bioactive component cinnamaldehyde show anti-tumor effects via inhibition of multiple cellular pathways
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237655/
https://www.ncbi.nlm.nih.gov/pubmed/35774603
http://dx.doi.org/10.3389/fphar.2022.918479
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