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Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa
BACKGROUND: Fungi use light as an environmental signal to regulate developmental transitions that are key aspects of their biological cycles and that are also relevant for their dispersal and infectivity as plant or animal pathogens. In addition, light regulates the accumulation of photoprotective p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238092/ https://www.ncbi.nlm.nih.gov/pubmed/35761233 http://dx.doi.org/10.1186/s12915-022-01351-x |
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author | Gil-Sánchez, María del Mar Cea-Sánchez, Sara Luque, Eva M. Cánovas, David Corrochano, Luis M. |
author_facet | Gil-Sánchez, María del Mar Cea-Sánchez, Sara Luque, Eva M. Cánovas, David Corrochano, Luis M. |
author_sort | Gil-Sánchez, María del Mar |
collection | PubMed |
description | BACKGROUND: Fungi use light as an environmental signal to regulate developmental transitions that are key aspects of their biological cycles and that are also relevant for their dispersal and infectivity as plant or animal pathogens. In addition, light regulates the accumulation of photoprotective pigments, like carotenoids, and other secondary metabolites. Most fungal light responses occur after changes in gene transcription and we describe here a novel effect of light in the regulation of degradation of VE-1, a key component of the velvet complex, in the model fungus Neurospora crassa. The velvet complex is a fungal-specific protein complex that coordinates fungal development, secondary metabolism, and light regulation by interacting with other regulators and photoreceptors and modifying gene expression. RESULTS: We have characterized the role of VE-1 during conidiation in N. crassa. In vegetative mycelia, VE-1 is localized in the cytoplasm and nuclei and is required for light-dependent transcription but does not interact with the photoreceptor and transcription factor WC-1. VE-1 is more stable in light than in darkness during asexual development (conidiation). We have shown that this light effect requires the blue-light photoreceptor WC-1. We have characterized the role of the proteasome, the COP9 signalosome (CSN), and the adaptor component of cullin-RING ubiquitin ligases, FWD-1, in the degradation of VE-1. CONCLUSIONS: We propose that this new effect of light allows the fungal cell to adapt quickly to changes in light exposure by promoting the accumulation of VE-1 for the regulation of genes that participate in the biosynthesis of photoprotective pigments. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01351-x. |
format | Online Article Text |
id | pubmed-9238092 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92380922022-06-29 Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa Gil-Sánchez, María del Mar Cea-Sánchez, Sara Luque, Eva M. Cánovas, David Corrochano, Luis M. BMC Biol Research Article BACKGROUND: Fungi use light as an environmental signal to regulate developmental transitions that are key aspects of their biological cycles and that are also relevant for their dispersal and infectivity as plant or animal pathogens. In addition, light regulates the accumulation of photoprotective pigments, like carotenoids, and other secondary metabolites. Most fungal light responses occur after changes in gene transcription and we describe here a novel effect of light in the regulation of degradation of VE-1, a key component of the velvet complex, in the model fungus Neurospora crassa. The velvet complex is a fungal-specific protein complex that coordinates fungal development, secondary metabolism, and light regulation by interacting with other regulators and photoreceptors and modifying gene expression. RESULTS: We have characterized the role of VE-1 during conidiation in N. crassa. In vegetative mycelia, VE-1 is localized in the cytoplasm and nuclei and is required for light-dependent transcription but does not interact with the photoreceptor and transcription factor WC-1. VE-1 is more stable in light than in darkness during asexual development (conidiation). We have shown that this light effect requires the blue-light photoreceptor WC-1. We have characterized the role of the proteasome, the COP9 signalosome (CSN), and the adaptor component of cullin-RING ubiquitin ligases, FWD-1, in the degradation of VE-1. CONCLUSIONS: We propose that this new effect of light allows the fungal cell to adapt quickly to changes in light exposure by promoting the accumulation of VE-1 for the regulation of genes that participate in the biosynthesis of photoprotective pigments. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12915-022-01351-x. BioMed Central 2022-06-27 /pmc/articles/PMC9238092/ /pubmed/35761233 http://dx.doi.org/10.1186/s12915-022-01351-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Gil-Sánchez, María del Mar Cea-Sánchez, Sara Luque, Eva M. Cánovas, David Corrochano, Luis M. Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa |
title | Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa |
title_full | Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa |
title_fullStr | Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa |
title_full_unstemmed | Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa |
title_short | Light regulates the degradation of the regulatory protein VE-1 in the fungus Neurospora crassa |
title_sort | light regulates the degradation of the regulatory protein ve-1 in the fungus neurospora crassa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238092/ https://www.ncbi.nlm.nih.gov/pubmed/35761233 http://dx.doi.org/10.1186/s12915-022-01351-x |
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