Visualizing the pH in Escherichia coli Colonies via the Sensor Protein mCherryEA Allows High-Throughput Screening of Mutant Libraries

Cytoplasmic pH in bacteria is tightly regulated by diverse active mechanisms and interconnected regulatory processes. Many processes and regulators underlying pH homeostasis have been identified via phenotypic screening of strain libraries for nongrowth at low or high pH values. Direct screens with...

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Detalles Bibliográficos
Autores principales: Hartmann, Fabian Stefan Franz, Weiß, Tamara, Shen, Jing, Smahajcsik, Dóra, Savickas, Simonas, Seibold, Gerd Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238402/
https://www.ncbi.nlm.nih.gov/pubmed/35430898
http://dx.doi.org/10.1128/msystems.00219-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9238402/
https://www.ncbi.nlm.nih.gov/pubmed/35430898
http://dx.doi.org/10.1128/msystems.00219-22

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