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Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes

Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes. Both of these processes are energized by the GldLM motor complex, which transduces the proton motive force at the inner membrane into mec...

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Autores principales: Hennell James, Rory, Deme, Justin C., Hunter, Alicia, Berks, Ben C., Lea, Susan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239094/
https://www.ncbi.nlm.nih.gov/pubmed/35446127
http://dx.doi.org/10.1128/mbio.00267-22
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author Hennell James, Rory
Deme, Justin C.
Hunter, Alicia
Berks, Ben C.
Lea, Susan M.
author_facet Hennell James, Rory
Deme, Justin C.
Hunter, Alicia
Berks, Ben C.
Lea, Susan M.
author_sort Hennell James, Rory
collection PubMed
description Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes. Both of these processes are energized by the GldLM motor complex, which transduces the proton motive force at the inner membrane into mechanical work at the outer membrane. We previously used cryo-electron microscopy to solve the structure of the GldLM motor core from Flavobacterium johnsoniae at 3.9-Å resolution (R. Hennell James, J. C. Deme, A. Kjaer, F. Alcock, et al., Nat Microbiol 6:221–233, 2021, https://dx.doi.org/10.1038/s41564-020-00823-6). Here, we present structures of homologous complexes from a range of pathogenic and environmental Bacteroidetes species at up to 3.0-Å resolution. These structures show that the architecture of the GldLM motor core is conserved across the Bacteroidetes phylum, although there are species-specific differences at the N terminus of GldL. The resolution improvements reveal a cage-like structure that ties together the membrane-proximal cytoplasmic region of GldL and influences gliding function. These findings add detail to our structural understanding of bacterial ion-driven motors that drive the T9SS and gliding motility.
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spelling pubmed-92390942022-06-29 Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes Hennell James, Rory Deme, Justin C. Hunter, Alicia Berks, Ben C. Lea, Susan M. mBio Research Article Gliding motility using cell surface adhesins, and export of proteins by the type IX secretion system (T9SS) are two phylum-specific features of the Bacteroidetes. Both of these processes are energized by the GldLM motor complex, which transduces the proton motive force at the inner membrane into mechanical work at the outer membrane. We previously used cryo-electron microscopy to solve the structure of the GldLM motor core from Flavobacterium johnsoniae at 3.9-Å resolution (R. Hennell James, J. C. Deme, A. Kjaer, F. Alcock, et al., Nat Microbiol 6:221–233, 2021, https://dx.doi.org/10.1038/s41564-020-00823-6). Here, we present structures of homologous complexes from a range of pathogenic and environmental Bacteroidetes species at up to 3.0-Å resolution. These structures show that the architecture of the GldLM motor core is conserved across the Bacteroidetes phylum, although there are species-specific differences at the N terminus of GldL. The resolution improvements reveal a cage-like structure that ties together the membrane-proximal cytoplasmic region of GldL and influences gliding function. These findings add detail to our structural understanding of bacterial ion-driven motors that drive the T9SS and gliding motility. American Society for Microbiology 2022-04-21 /pmc/articles/PMC9239094/ /pubmed/35446127 http://dx.doi.org/10.1128/mbio.00267-22 Text en https://doi.org/10.1128/AuthorWarrantyLicense.v1This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
spellingShingle Research Article
Hennell James, Rory
Deme, Justin C.
Hunter, Alicia
Berks, Ben C.
Lea, Susan M.
Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
title Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
title_full Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
title_fullStr Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
title_full_unstemmed Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
title_short Structures of the Type IX Secretion/Gliding Motility Motor from across the Phylum Bacteroidetes
title_sort structures of the type ix secretion/gliding motility motor from across the phylum bacteroidetes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239094/
https://www.ncbi.nlm.nih.gov/pubmed/35446127
http://dx.doi.org/10.1128/mbio.00267-22
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