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Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae

Mutations in regulatory mechanisms that control gene expression contribute to phenotypic diversity and thus facilitate the adaptation of microbes and other organisms to new niches. Comparative genomics can be used to infer rewiring of regulatory architecture based on large effect mutations like loss...

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Autores principales: Ng, Siu Lung, Kammann, Sophia, Steinbach, Gabi, Hoffmann, Tobias, Yunker, Peter J., Hammer, Brian K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239110/
https://www.ncbi.nlm.nih.gov/pubmed/35604123
http://dx.doi.org/10.1128/mbio.00422-22
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author Ng, Siu Lung
Kammann, Sophia
Steinbach, Gabi
Hoffmann, Tobias
Yunker, Peter J.
Hammer, Brian K.
author_facet Ng, Siu Lung
Kammann, Sophia
Steinbach, Gabi
Hoffmann, Tobias
Yunker, Peter J.
Hammer, Brian K.
author_sort Ng, Siu Lung
collection PubMed
description Mutations in regulatory mechanisms that control gene expression contribute to phenotypic diversity and thus facilitate the adaptation of microbes and other organisms to new niches. Comparative genomics can be used to infer rewiring of regulatory architecture based on large effect mutations like loss or acquisition of transcription factors but may be insufficient to identify small changes in noncoding, intergenic DNA sequence of regulatory elements that drive phenotypic divergence. In human-derived Vibrio cholerae, the response to distinct chemical cues triggers production of multiple transcription factors that can regulate the type VI secretion system (T6), a broadly distributed weapon for interbacterial competition. However, to date, the signaling network remains poorly understood because no regulatory element has been identified for the major T6 locus. Here we identify a conserved cis-acting single nucleotide polymorphism (SNP) controlling T6 transcription and activity. Sequence alignment of the T6 regulatory region from diverse V. cholerae strains revealed conservation of the SNP that we rewired to interconvert V. cholerae T6 activity between chitin-inducible and constitutive states. This study supports a model of pathogen evolution through a noncoding cis-regulatory mutation and preexisting, active transcription factors that confers a different fitness advantage to tightly regulated strains inside a human host and unfettered strains adapted to environmental niches.
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spelling pubmed-92391102022-06-29 Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae Ng, Siu Lung Kammann, Sophia Steinbach, Gabi Hoffmann, Tobias Yunker, Peter J. Hammer, Brian K. mBio Research Article Mutations in regulatory mechanisms that control gene expression contribute to phenotypic diversity and thus facilitate the adaptation of microbes and other organisms to new niches. Comparative genomics can be used to infer rewiring of regulatory architecture based on large effect mutations like loss or acquisition of transcription factors but may be insufficient to identify small changes in noncoding, intergenic DNA sequence of regulatory elements that drive phenotypic divergence. In human-derived Vibrio cholerae, the response to distinct chemical cues triggers production of multiple transcription factors that can regulate the type VI secretion system (T6), a broadly distributed weapon for interbacterial competition. However, to date, the signaling network remains poorly understood because no regulatory element has been identified for the major T6 locus. Here we identify a conserved cis-acting single nucleotide polymorphism (SNP) controlling T6 transcription and activity. Sequence alignment of the T6 regulatory region from diverse V. cholerae strains revealed conservation of the SNP that we rewired to interconvert V. cholerae T6 activity between chitin-inducible and constitutive states. This study supports a model of pathogen evolution through a noncoding cis-regulatory mutation and preexisting, active transcription factors that confers a different fitness advantage to tightly regulated strains inside a human host and unfettered strains adapted to environmental niches. American Society for Microbiology 2022-05-23 /pmc/articles/PMC9239110/ /pubmed/35604123 http://dx.doi.org/10.1128/mbio.00422-22 Text en Copyright © 2022 Ng et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Ng, Siu Lung
Kammann, Sophia
Steinbach, Gabi
Hoffmann, Tobias
Yunker, Peter J.
Hammer, Brian K.
Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae
title Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae
title_full Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae
title_fullStr Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae
title_full_unstemmed Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae
title_short Evolution of a cis-Acting SNP That Controls Type VI Secretion in Vibrio cholerae
title_sort evolution of a cis-acting snp that controls type vi secretion in vibrio cholerae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239110/
https://www.ncbi.nlm.nih.gov/pubmed/35604123
http://dx.doi.org/10.1128/mbio.00422-22
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