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The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season
Leaves are primarily responsible for the plant’s photosynthetic activity. Thus, changes in the leaf microbiota, which includes deleterious and beneficial microbes, can have far-reaching effects on plant fitness and productivity. Identifying the processes and microorganisms that drive these changes o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239250/ https://www.ncbi.nlm.nih.gov/pubmed/35420486 http://dx.doi.org/10.1128/mbio.02825-21 |
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author | Almario, Juliana Mahmoudi, Maryam Kroll, Samuel Agler, Mathew Placzek, Aleksandra Mari, Alfredo Kemen, Eric |
author_facet | Almario, Juliana Mahmoudi, Maryam Kroll, Samuel Agler, Mathew Placzek, Aleksandra Mari, Alfredo Kemen, Eric |
author_sort | Almario, Juliana |
collection | PubMed |
description | Leaves are primarily responsible for the plant’s photosynthetic activity. Thus, changes in the leaf microbiota, which includes deleterious and beneficial microbes, can have far-reaching effects on plant fitness and productivity. Identifying the processes and microorganisms that drive these changes over a plant’s lifetime is, therefore, crucial. In this study, we analyzed the temporal dynamics in the leaf microbiome of Arabidopsis thaliana, integrating changes in both composition and microbe-microbe interactions via the study of microbial networks. Field-grown Arabidopsis were used to monitor leaf bacterial, fungal and oomycete communities throughout the plant’s natural growing season (extending from November to March) over three consecutive years. Our results revealed the existence of conserved temporal patterns, with microbial communities and networks going through a stabilization phase of decreased diversity and variability at the beginning of the plant’s growing season. Despite a high turnover in these communities, we identified 19 “core” taxa persisting on Arabidopsis leaves across time and plant generations. With the hypothesis these microbes could be playing key roles in the structuring of leaf microbial communities, we conducted a time-informed microbial network analysis which showed core taxa are not necessarily highly connected network “hubs,” and “hubs” alternate with time. Our study shows that leaf microbial communities exhibit reproducible dynamics and patterns, suggesting the potential of using our understanding of temporal trajectories in microbial community composition to design experiments aimed at driving these communities toward desired states. |
format | Online Article Text |
id | pubmed-9239250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-92392502022-06-29 The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season Almario, Juliana Mahmoudi, Maryam Kroll, Samuel Agler, Mathew Placzek, Aleksandra Mari, Alfredo Kemen, Eric mBio Research Article Leaves are primarily responsible for the plant’s photosynthetic activity. Thus, changes in the leaf microbiota, which includes deleterious and beneficial microbes, can have far-reaching effects on plant fitness and productivity. Identifying the processes and microorganisms that drive these changes over a plant’s lifetime is, therefore, crucial. In this study, we analyzed the temporal dynamics in the leaf microbiome of Arabidopsis thaliana, integrating changes in both composition and microbe-microbe interactions via the study of microbial networks. Field-grown Arabidopsis were used to monitor leaf bacterial, fungal and oomycete communities throughout the plant’s natural growing season (extending from November to March) over three consecutive years. Our results revealed the existence of conserved temporal patterns, with microbial communities and networks going through a stabilization phase of decreased diversity and variability at the beginning of the plant’s growing season. Despite a high turnover in these communities, we identified 19 “core” taxa persisting on Arabidopsis leaves across time and plant generations. With the hypothesis these microbes could be playing key roles in the structuring of leaf microbial communities, we conducted a time-informed microbial network analysis which showed core taxa are not necessarily highly connected network “hubs,” and “hubs” alternate with time. Our study shows that leaf microbial communities exhibit reproducible dynamics and patterns, suggesting the potential of using our understanding of temporal trajectories in microbial community composition to design experiments aimed at driving these communities toward desired states. American Society for Microbiology 2022-04-14 /pmc/articles/PMC9239250/ /pubmed/35420486 http://dx.doi.org/10.1128/mbio.02825-21 Text en Copyright © 2022 Almario et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Almario, Juliana Mahmoudi, Maryam Kroll, Samuel Agler, Mathew Placzek, Aleksandra Mari, Alfredo Kemen, Eric The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season |
title | The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season |
title_full | The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season |
title_fullStr | The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season |
title_full_unstemmed | The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season |
title_short | The Leaf Microbiome of Arabidopsis Displays Reproducible Dynamics and Patterns throughout the Growing Season |
title_sort | leaf microbiome of arabidopsis displays reproducible dynamics and patterns throughout the growing season |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239250/ https://www.ncbi.nlm.nih.gov/pubmed/35420486 http://dx.doi.org/10.1128/mbio.02825-21 |
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