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Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin
[Image: see text] COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysac...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239546/ https://www.ncbi.nlm.nih.gov/pubmed/35774232 http://dx.doi.org/10.1021/acsptsci.1c00219 |
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author | Puthia, Manoj Tanner, Lloyd Petruk, Ganna Schmidtchen, Artur |
author_facet | Puthia, Manoj Tanner, Lloyd Petruk, Ganna Schmidtchen, Artur |
author_sort | Puthia, Manoj |
collection | PubMed |
description | [Image: see text] COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysaccharide (LPS), leading to the boosting of inflammation. Here we present a mouse inflammation model employing the coadministration of aerosolized S protein together with LPS to the lungs. Using NF-κB-RE-Luc reporter and C57BL/6 mice followed by combinations of bioimaging, cytokine, chemokine, fluorescence-activated cell sorting, and histochemistry analyses, we show that the model yields severe pulmonary inflammation and a cytokine profile similar to that observed in COVID-19. Therefore, the model offers utility for analyses of the pathophysiological features of COVID-19 and the development of new treatments. |
format | Online Article Text |
id | pubmed-9239546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-92395462023-01-25 Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin Puthia, Manoj Tanner, Lloyd Petruk, Ganna Schmidtchen, Artur ACS Pharmacol Transl Sci [Image: see text] COVID-19 is characterized by a dysregulated and excessive inflammatory response and, in severe cases, acute respiratory distress syndrome. We have recently demonstrated a previously unknown high-affinity interaction between the SARS-CoV-2 spike (S) protein and bacterial lipopolysaccharide (LPS), leading to the boosting of inflammation. Here we present a mouse inflammation model employing the coadministration of aerosolized S protein together with LPS to the lungs. Using NF-κB-RE-Luc reporter and C57BL/6 mice followed by combinations of bioimaging, cytokine, chemokine, fluorescence-activated cell sorting, and histochemistry analyses, we show that the model yields severe pulmonary inflammation and a cytokine profile similar to that observed in COVID-19. Therefore, the model offers utility for analyses of the pathophysiological features of COVID-19 and the development of new treatments. American Chemical Society 2022-01-25 /pmc/articles/PMC9239546/ /pubmed/35774232 http://dx.doi.org/10.1021/acsptsci.1c00219 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Puthia, Manoj Tanner, Lloyd Petruk, Ganna Schmidtchen, Artur Experimental Model of Pulmonary Inflammation Induced by SARS-CoV-2 Spike Protein and Endotoxin |
title | Experimental Model of Pulmonary Inflammation Induced
by SARS-CoV-2 Spike Protein and Endotoxin |
title_full | Experimental Model of Pulmonary Inflammation Induced
by SARS-CoV-2 Spike Protein and Endotoxin |
title_fullStr | Experimental Model of Pulmonary Inflammation Induced
by SARS-CoV-2 Spike Protein and Endotoxin |
title_full_unstemmed | Experimental Model of Pulmonary Inflammation Induced
by SARS-CoV-2 Spike Protein and Endotoxin |
title_short | Experimental Model of Pulmonary Inflammation Induced
by SARS-CoV-2 Spike Protein and Endotoxin |
title_sort | experimental model of pulmonary inflammation induced
by sars-cov-2 spike protein and endotoxin |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239546/ https://www.ncbi.nlm.nih.gov/pubmed/35774232 http://dx.doi.org/10.1021/acsptsci.1c00219 |
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