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Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses

Grapevine is one of the most cultivated fruit plant among economically relevant species in the world. It is vegetatively propagated and can be attacked by more than 80 viruses with possible detrimental effects on crop yield and wine quality. Preventive measures relying on extensive and robust diagno...

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Autores principales: Mandrile, Luisa, D’Errico, Chiara, Nuzzo, Floriana, Barzan, Giulia, Matić, Slavica, Giovannozzi, Andrea M., Rossi, Andrea M., Gambino, Giorgio, Noris, Emanuela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239551/
https://www.ncbi.nlm.nih.gov/pubmed/35774819
http://dx.doi.org/10.3389/fpls.2022.917226
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author Mandrile, Luisa
D’Errico, Chiara
Nuzzo, Floriana
Barzan, Giulia
Matić, Slavica
Giovannozzi, Andrea M.
Rossi, Andrea M.
Gambino, Giorgio
Noris, Emanuela
author_facet Mandrile, Luisa
D’Errico, Chiara
Nuzzo, Floriana
Barzan, Giulia
Matić, Slavica
Giovannozzi, Andrea M.
Rossi, Andrea M.
Gambino, Giorgio
Noris, Emanuela
author_sort Mandrile, Luisa
collection PubMed
description Grapevine is one of the most cultivated fruit plant among economically relevant species in the world. It is vegetatively propagated and can be attacked by more than 80 viruses with possible detrimental effects on crop yield and wine quality. Preventive measures relying on extensive and robust diagnosis are fundamental to guarantee the use of virus-free grapevine plants and to manage its diseases. New phenotyping techniques for non-invasive identification of biochemical changes occurring during virus infection can be used for rapid diagnostic purposes. Here, we have investigated the potential of Raman spectroscopy (RS) to identify the presence of two different viruses, grapevine fan leaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) in Vitis vinifera cv. Chardonnay. We showed that RS can discriminate healthy plants from those infected by each of the two viruses, even in the absence of visible symptoms, with accuracy up to 100% and 80% for GFLV and GRSPaV, respectively. Chemometric analyses of the Raman spectra followed by chemical measurements showed that RS could probe a decrease in the carotenoid content in infected leaves, more profoundly altered by GFLV infection. Transcriptional analysis of genes involved in the carotenoid pathway confirmed that this biosynthetic process is altered during infection. These results indicate that RS is a cutting-edge alternative for a real-time dynamic monitoring of pathogens in grapevine plants and can be useful for studying the metabolic changes ensuing from plant stresses.
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spelling pubmed-92395512022-06-29 Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses Mandrile, Luisa D’Errico, Chiara Nuzzo, Floriana Barzan, Giulia Matić, Slavica Giovannozzi, Andrea M. Rossi, Andrea M. Gambino, Giorgio Noris, Emanuela Front Plant Sci Plant Science Grapevine is one of the most cultivated fruit plant among economically relevant species in the world. It is vegetatively propagated and can be attacked by more than 80 viruses with possible detrimental effects on crop yield and wine quality. Preventive measures relying on extensive and robust diagnosis are fundamental to guarantee the use of virus-free grapevine plants and to manage its diseases. New phenotyping techniques for non-invasive identification of biochemical changes occurring during virus infection can be used for rapid diagnostic purposes. Here, we have investigated the potential of Raman spectroscopy (RS) to identify the presence of two different viruses, grapevine fan leaf virus (GFLV) and grapevine rupestris stem pitting-associated virus (GRSPaV) in Vitis vinifera cv. Chardonnay. We showed that RS can discriminate healthy plants from those infected by each of the two viruses, even in the absence of visible symptoms, with accuracy up to 100% and 80% for GFLV and GRSPaV, respectively. Chemometric analyses of the Raman spectra followed by chemical measurements showed that RS could probe a decrease in the carotenoid content in infected leaves, more profoundly altered by GFLV infection. Transcriptional analysis of genes involved in the carotenoid pathway confirmed that this biosynthetic process is altered during infection. These results indicate that RS is a cutting-edge alternative for a real-time dynamic monitoring of pathogens in grapevine plants and can be useful for studying the metabolic changes ensuing from plant stresses. Frontiers Media S.A. 2022-06-14 /pmc/articles/PMC9239551/ /pubmed/35774819 http://dx.doi.org/10.3389/fpls.2022.917226 Text en Copyright © 2022 Mandrile, D’Errico, Nuzzo, Barzan, Matić, Giovannozzi, Rossi, Gambino and Noris. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Mandrile, Luisa
D’Errico, Chiara
Nuzzo, Floriana
Barzan, Giulia
Matić, Slavica
Giovannozzi, Andrea M.
Rossi, Andrea M.
Gambino, Giorgio
Noris, Emanuela
Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses
title Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses
title_full Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses
title_fullStr Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses
title_full_unstemmed Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses
title_short Raman Spectroscopy Applications in Grapevine: Metabolic Analysis of Plants Infected by Two Different Viruses
title_sort raman spectroscopy applications in grapevine: metabolic analysis of plants infected by two different viruses
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239551/
https://www.ncbi.nlm.nih.gov/pubmed/35774819
http://dx.doi.org/10.3389/fpls.2022.917226
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