An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination

Previously, we described a large collection of Drosophila strains that each carry an artificial exon containing a T2AGAL4 cassette inserted in an intron of a target gene based on CRISPR-mediated homologous recombination. These alleles permit numerous applications and have proven to be very useful. I...

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Detalles Bibliográficos
Autores principales: Kanca, Oguz, Zirin, Jonathan, Hu, Yanhui, Tepe, Burak, Dutta, Debdeep, Lin, Wen-Wen, Ma, Liwen, Ge, Ming, Zuo, Zhongyuan, Liu, Lu-Ping, Levis, Robert W, Perrimon, Norbert, Bellen, Hugo J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Genetics and Genomics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239680/
https://www.ncbi.nlm.nih.gov/pubmed/35723254
http://dx.doi.org/10.7554/eLife.76077

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