Cargando…

Factors associated with weak positive SARS-CoV-2 diagnosis by reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR)

During the COVID-19 pandemic, the reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) assay has been the primary method of diagnosis of SARS-CoV-2 infection. However, RT-qPCR assay interpretation can be ambiguous with no universal absolute cut-off value to determine sample positiv...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rawat, Priyank, Zerbato, Jennifer M., Rhodes, Ajantha, Chiu, Chris, Tran, Thomas, Rasmussen, Thomas A., Druce, Julian, Lewin, Sharon R., Roche, Michael
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal College of Pathologists of Australasia. Published by Elsevier B.V. 2022
Materias:	Virology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239708/ https://www.ncbi.nlm.nih.gov/pubmed/35778288 http://dx.doi.org/10.1016/j.pathol.2022.04.001

Ejemplares similares

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19
por: Nagura-Ikeda, Mayu, et al.
Publicado: (2020)

Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I
por: OKINO, Cintia Hiromi, et al.
Publicado: (2018)

Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America()()
por: Edwards, Thomas, et al.
Publicado: (2017)

Development and Evaluation of an Enterovirus D68 Real-Time Reverse Transcriptase PCR Assay
por: Wylie, Todd N., et al.
Publicado: (2015)

Development of a Rapid Live SARS-CoV-2 Neutralization Assay Based on a qPCR Readout
por: Lichtenegger, Sabine, et al.
Publicado: (2022)

Utility of a Stressed Single Nucleotide Polymorphism (SNP) Real-Time PCR Assay for Rapid Identification of Measles Vaccine Strains in Patient Samples
por: Tran, Thomas, et al.
Publicado: (2018)

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
por: LIU, Dafei, et al.
Publicado: (2018)

Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology
por: Belák, S., et al.
Publicado: (1993)

A Simple Reverse Transcriptase PCR Melting-Temperature Assay To Rapidly Screen for Widely Circulating SARS-CoV-2 Variants
por: Banada, Padmapriya, et al.
Publicado: (2021)

Impact of SARS-CoV-2 Variants on the Analytical Sensitivity of rRT-PCR Assays
por: Chen, Yuqing, et al.
Publicado: (2022)

Portable RT-PCR System: a Rapid and Scalable Diagnostic Tool for COVID-19 Testing
por: Zowawi, Hosam M., et al.
Publicado: (2021)

Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection
por: TAKANO, Tomomi, et al.
Publicado: (2021)

Detection of Bovine Group A Rotavirus Using Rapid Antigen Detection Kits, RT-PCR and Next-Generation DNA Sequencing
por: MINAMI-FUKUDA, Fujiko, et al.
Publicado: (2013)

Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2
por: Moore, Nicholas M., et al.
Publicado: (2020)

Performance Characteristics of the Abbott BinaxNOW SARS-CoV-2 Antigen Test in Comparison to Real-Time Reverse Transcriptase PCR and Viral Culture in Community Testing Sites during November 2020
por: Almendares, Olivia, et al.
Publicado: (2022)

Application of a SYBR® Green One Step Real-time RT-PCR Assay to Detect Type 1 Porcine Reproductive and Respiratory Syndrome Virus
por: ISEKI, Hiroshi, et al.
Publicado: (2014)

Development of a quantitative real-time RT-PCR assay for detecting Taiwan ferret badger rabies virus in ear tissue of ferret badgers and mice
por: HSU, Ai-Ping, et al.
Publicado: (2018)

Use of quantitative real-time RT-PCR to investigate the correlation between viremia and viral shedding of canine distemper virus, and infection outcomes in experimentally infected dogs
por: SEHATA, Go, et al.
Publicado: (2015)

Development and evaluation of multiplex real-time RT-PCR assays for seasonal, pandemic A/H1pdm09 and avian A/H5 influenza viruses detection
por: Choi, Jang-Hoon, et al.
Publicado: (2013)

Patterns of hepatitis B virus S gene escape mutants and reverse transcriptase mutations among genotype D isolates in Jordan
por: Ababneh, Nidaa A., et al.
Publicado: (2019)

SARS-CoV-2 viral load dynamics and real-time RT-PCR cycle threshold interpretation in symptomatic non-hospitalised individuals in New Zealand: a multicentre cross sectional observational study
por: Fox-Lewis, Andrew, et al.
Publicado: (2021)

Characterization and Clinical Significance of Natural Variability in Hepatitis B Virus Reverse Transcriptase in Treatment-Naive Chinese Patients by Sanger Sequencing and Next-Generation Sequencing
por: Fu, Ya, et al.
Publicado: (2019)

The Agreement Between Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and Rapid Antigen Test (RAT) in Diagnosing COVID-19
por: Sahu, Rupesh, et al.
Publicado: (2022)

Viral detection using a multiplex polymerase chain reaction–based assay in outpatients with upper respiratory infection()
por: Leekha, Surbhi, et al.
Publicado: (2013)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

Quantitative Evaluation of Very Low Levels of HIV-1 Reverse Transcriptase by a Novel Highly Sensitive RT-qPCR Assay
por: Marino-Merlo, Francesca, et al.
Publicado: (2022)

Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater
por: Ciesielski, Mark, et al.
Publicado: (2021)

Quantitative PCR detection of feline morbillivirus in cat urine samples
por: FURUYA, Tetsuya, et al.
Publicado: (2015)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Direct polymerase chain reaction from blood and tissue samples for rapid diagnosis of bovine leukemia virus infection
por: NISHIMORI, Asami, et al.
Publicado: (2016)

Comparison of RT-qPCR and RT-ddPCR with Rift valley fever virus (RVFV) RNA
por: Park, Changwoo, et al.
Publicado: (2023)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES
por: STAGGEMEIER, Rodrigo, et al.
Publicado: (2015)

Utility of Stool PCR for the Diagnosis of COVID-19: Comparison of Two Commercial Platforms
por: Szymczak, Wendy A., et al.
Publicado: (2020)

SASqPCR: Robust and Rapid Analysis of RT-qPCR Data in SAS
por: Ling, Daijun
Publicado: (2012)

Appraisal of a Simple and Effective RT-qPCR Assay for Evaluating the Reverse Transcriptase Activity in Blood Samples from HIV-1 Patients
por: Macchi, Beatrice, et al.
Publicado: (2020)

Reverse transcription polymerase chain reaction and electrospray ionization mass spectrometry for identifying acute viral upper respiratory tract infections()()()
por: Chen, Kuan-Fu, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_vlnsee91pf2sbt9bsq8it1sen6