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The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma
BACKGROUND: Asthma is known as a disease that causes breathing problems in children and adults and is also associated with chronic inflammation and oxidative stress of the airways. Nasturtium officinale (NO) possesses a wide range of pharmacological properties, particularly anti-inflammation and ant...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239788/ https://www.ncbi.nlm.nih.gov/pubmed/35774748 http://dx.doi.org/10.1155/2022/5319237 |
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author | shakerinasab, Nasrin Bejeshk, Mohammad Abbas Pourghadamyari, Hossein Najafipour, Hamid Eftekhari, Mahdieh Mottaghipisheh, Javad Omidifar, Navid Azizi, Mahdokht Rajizadeh, Mohammad Amin Doustimotlagh, Amir Hossein |
author_facet | shakerinasab, Nasrin Bejeshk, Mohammad Abbas Pourghadamyari, Hossein Najafipour, Hamid Eftekhari, Mahdieh Mottaghipisheh, Javad Omidifar, Navid Azizi, Mahdokht Rajizadeh, Mohammad Amin Doustimotlagh, Amir Hossein |
author_sort | shakerinasab, Nasrin |
collection | PubMed |
description | BACKGROUND: Asthma is known as a disease that causes breathing problems in children and adults and is also associated with chronic inflammation and oxidative stress of the airways. Nasturtium officinale (NO) possesses a wide range of pharmacological properties, particularly anti-inflammation and antioxidant potentials. Thus, this study for the first time was aimed to investigate anti-inflammatory and antioxidative activities of NO extract (NOE) in an ovalbumin-induced rat model of asthma. MATERIALS AND METHODS: Forty-four male Wistar rats were sensitized with ovalbumin (OVA) to induce asthma symptoms. The animals were allocated into five groups: control (C), asthmatic (A), A + NOE (500 mg/kg), NOE (500 mg/kg), and A + dexamethasone (DX, 2.5 mg/kg). After 7 days, blood and tissue samples were taken from the rats. Then, the level of inflammatory markers, oxidative stress parameters, and antioxidant enzymes activity were measured. RESULTS: The obtained results showed that OVA-sensitive rats significantly increased the levels of pro-inflammatory cytokines IL-1B, TGF-β, and SMA-α compared to the control group (p < 0.05), while treatment with NOE remarkably reduced the SMA-α gene expression compared to the asthma group (p < 0.05). Furthermore, it decreased the expression of IL-1B and TNF-α genes, although it was not statistically significant. The level of glutathione peroxidase (GPX) significantly reduced in A group compared to the C group (p < 0.05), whereas NOE administration significantly increased this marker (p < 0.05). Moreover, NOE attenuated inflammation and alveolar injury in the lungs of OVA-sensitive rat compared to the nontreated A group. CONCLUSIONS: Overall, our findings demonstrated that NOE somewhat is able to reduce airway inflammation by reducing inflammatory and increasing GPX activity. Indeed, further experiments investigating the impact of different extract doses are needed to confirm the antioxidant and anti-inflammatory effects of NOE. |
format | Online Article Text |
id | pubmed-9239788 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-92397882022-06-29 The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma shakerinasab, Nasrin Bejeshk, Mohammad Abbas Pourghadamyari, Hossein Najafipour, Hamid Eftekhari, Mahdieh Mottaghipisheh, Javad Omidifar, Navid Azizi, Mahdokht Rajizadeh, Mohammad Amin Doustimotlagh, Amir Hossein Evid Based Complement Alternat Med Research Article BACKGROUND: Asthma is known as a disease that causes breathing problems in children and adults and is also associated with chronic inflammation and oxidative stress of the airways. Nasturtium officinale (NO) possesses a wide range of pharmacological properties, particularly anti-inflammation and antioxidant potentials. Thus, this study for the first time was aimed to investigate anti-inflammatory and antioxidative activities of NO extract (NOE) in an ovalbumin-induced rat model of asthma. MATERIALS AND METHODS: Forty-four male Wistar rats were sensitized with ovalbumin (OVA) to induce asthma symptoms. The animals were allocated into five groups: control (C), asthmatic (A), A + NOE (500 mg/kg), NOE (500 mg/kg), and A + dexamethasone (DX, 2.5 mg/kg). After 7 days, blood and tissue samples were taken from the rats. Then, the level of inflammatory markers, oxidative stress parameters, and antioxidant enzymes activity were measured. RESULTS: The obtained results showed that OVA-sensitive rats significantly increased the levels of pro-inflammatory cytokines IL-1B, TGF-β, and SMA-α compared to the control group (p < 0.05), while treatment with NOE remarkably reduced the SMA-α gene expression compared to the asthma group (p < 0.05). Furthermore, it decreased the expression of IL-1B and TNF-α genes, although it was not statistically significant. The level of glutathione peroxidase (GPX) significantly reduced in A group compared to the C group (p < 0.05), whereas NOE administration significantly increased this marker (p < 0.05). Moreover, NOE attenuated inflammation and alveolar injury in the lungs of OVA-sensitive rat compared to the nontreated A group. CONCLUSIONS: Overall, our findings demonstrated that NOE somewhat is able to reduce airway inflammation by reducing inflammatory and increasing GPX activity. Indeed, further experiments investigating the impact of different extract doses are needed to confirm the antioxidant and anti-inflammatory effects of NOE. Hindawi 2022-06-21 /pmc/articles/PMC9239788/ /pubmed/35774748 http://dx.doi.org/10.1155/2022/5319237 Text en Copyright © 2022 Nasrin shakerinasab et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article shakerinasab, Nasrin Bejeshk, Mohammad Abbas Pourghadamyari, Hossein Najafipour, Hamid Eftekhari, Mahdieh Mottaghipisheh, Javad Omidifar, Navid Azizi, Mahdokht Rajizadeh, Mohammad Amin Doustimotlagh, Amir Hossein The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma |
title | The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma |
title_full | The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma |
title_fullStr | The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma |
title_full_unstemmed | The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma |
title_short | The Hydroalcoholic Extract of Nasturtium officinale Reduces Lung Inflammation and Oxidative Stress in an Ovalbumin-Induced Rat Model of Asthma |
title_sort | hydroalcoholic extract of nasturtium officinale reduces lung inflammation and oxidative stress in an ovalbumin-induced rat model of asthma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239788/ https://www.ncbi.nlm.nih.gov/pubmed/35774748 http://dx.doi.org/10.1155/2022/5319237 |
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