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Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC
The purpose of this study was to investigate the structural characterization and immunological activity in vitro and in vivo of a polysaccharide from the rhizome of Menispermum dauricum. A new polysaccharide named MDP was isolated from the rhizome of Menispermum dauricum by hot water extraction, eth...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240474/ https://www.ncbi.nlm.nih.gov/pubmed/35782915 http://dx.doi.org/10.3389/fnut.2022.922569 |
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author | Yang, Pei Jin, Juan Ma, Yan Wang, Fengshan Li, Yaying Duan, Baoguo Zhang, Yongqing Liu, Yuhong |
author_facet | Yang, Pei Jin, Juan Ma, Yan Wang, Fengshan Li, Yaying Duan, Baoguo Zhang, Yongqing Liu, Yuhong |
author_sort | Yang, Pei |
collection | PubMed |
description | The purpose of this study was to investigate the structural characterization and immunological activity in vitro and in vivo of a polysaccharide from the rhizome of Menispermum dauricum. A new polysaccharide named MDP was isolated from the rhizome of Menispermum dauricum by hot water extraction, ethanol precipitation, anion-exchange, and gel-filtration chromatography. MDP was homogeneous and had a molecular weight of 6.16 ×10(3) Da, and it was an α-D-glucan containing a (1 → 6)-linked backbone, with a glucosyl residue at the C-3 position along the main chain. MDP exhibited immunological activity in vitro, which could significantly promote the proliferation and phagocytosis of RAW264.7 cells and the release of TNF-α and IL-6 factors. For immunological activity in vivo. MDP could significantly increase the thymus and spleen indices, enhance the macrophage function, increase the level of cytokine (IL-6 and TNF-α) and immunoglobulin IgM in the serum and regulate T lymphocyte subsets. Furthermore, MDP elevated the expression of the critical nodes in the TLR4-MyD88 signaling pathways in vivo. These results support the concept that MDP may exhibit immunological activity through TLR4-MyD88 signaling pathway in vivo. |
format | Online Article Text |
id | pubmed-9240474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92404742022-06-30 Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC Yang, Pei Jin, Juan Ma, Yan Wang, Fengshan Li, Yaying Duan, Baoguo Zhang, Yongqing Liu, Yuhong Front Nutr Nutrition The purpose of this study was to investigate the structural characterization and immunological activity in vitro and in vivo of a polysaccharide from the rhizome of Menispermum dauricum. A new polysaccharide named MDP was isolated from the rhizome of Menispermum dauricum by hot water extraction, ethanol precipitation, anion-exchange, and gel-filtration chromatography. MDP was homogeneous and had a molecular weight of 6.16 ×10(3) Da, and it was an α-D-glucan containing a (1 → 6)-linked backbone, with a glucosyl residue at the C-3 position along the main chain. MDP exhibited immunological activity in vitro, which could significantly promote the proliferation and phagocytosis of RAW264.7 cells and the release of TNF-α and IL-6 factors. For immunological activity in vivo. MDP could significantly increase the thymus and spleen indices, enhance the macrophage function, increase the level of cytokine (IL-6 and TNF-α) and immunoglobulin IgM in the serum and regulate T lymphocyte subsets. Furthermore, MDP elevated the expression of the critical nodes in the TLR4-MyD88 signaling pathways in vivo. These results support the concept that MDP may exhibit immunological activity through TLR4-MyD88 signaling pathway in vivo. Frontiers Media S.A. 2022-06-15 /pmc/articles/PMC9240474/ /pubmed/35782915 http://dx.doi.org/10.3389/fnut.2022.922569 Text en Copyright © 2022 Yang, Jin, Ma, Wang, Li, Duan, Zhang and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Yang, Pei Jin, Juan Ma, Yan Wang, Fengshan Li, Yaying Duan, Baoguo Zhang, Yongqing Liu, Yuhong Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC |
title | Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC |
title_full | Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC |
title_fullStr | Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC |
title_full_unstemmed | Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC |
title_short | Structure Characterization, Immunological Activity, and Mechanism of a Polysaccharide From the Rhizome of Menispermum dauricum DC |
title_sort | structure characterization, immunological activity, and mechanism of a polysaccharide from the rhizome of menispermum dauricum dc |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240474/ https://www.ncbi.nlm.nih.gov/pubmed/35782915 http://dx.doi.org/10.3389/fnut.2022.922569 |
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