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Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
In recent decades, infections caused by the opportunistic fungus Candida albicans have increased, especially in patients with immunodeficiency. In this study, we investigated the mechanism of action of sanguinarine (SAN) against C. albicans both in vitro and in vivo. SAN exhibited antifungal activit...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240711/ https://www.ncbi.nlm.nih.gov/pubmed/35783394 http://dx.doi.org/10.3389/fmicb.2022.908461 |
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author | Hu, Ziwei Hu, Hao Hu, Zhili Zhong, Xiaojun Guan, Yifu Zhao, Yunshi Wang, Lu Ye, Liang Ming, Liliang Riaz Rajoka, Muhammad Shahid He, Zhendan Wang, Yan Song, Xun |
author_facet | Hu, Ziwei Hu, Hao Hu, Zhili Zhong, Xiaojun Guan, Yifu Zhao, Yunshi Wang, Lu Ye, Liang Ming, Liliang Riaz Rajoka, Muhammad Shahid He, Zhendan Wang, Yan Song, Xun |
author_sort | Hu, Ziwei |
collection | PubMed |
description | In recent decades, infections caused by the opportunistic fungus Candida albicans have increased, especially in patients with immunodeficiency. In this study, we investigated the mechanism of action of sanguinarine (SAN) against C. albicans both in vitro and in vivo. SAN exhibited antifungal activity against C. albicans clinical isolates, with MICs in the range of 112.8–150.5 μM. Furthermore, scanning electron and transmission electron microscopy showed that SAN induced morphological changes as well as structure disruption in C. albicans cells, including masses of cellular debris, ruptured cell walls, and membrane deformation. Flow cytometry revealed that SAN could lead to cell membrane damage, and ergosterol content analysis indicated that SAN could cause ergosterol content reduction exceeding 90%. Further, we validated the efficacy of SAN against candidiasis caused by C. albicans in a murine model, and SAN significantly improved survival and reduced weight loss compared to vehicle. The treatment of 1.5 and 2.5 mg/kg/d SAN obviously reduced the fungal burden in the kidney. In addition, histopathological examination indicated that no fungal cells were observed in lung and kidney tissues after SAN treatment. Hence, this study suggests that SAN is a promising plant-derived compound for the development of an effective anticandidal agent. |
format | Online Article Text |
id | pubmed-9240711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92407112022-06-30 Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis Hu, Ziwei Hu, Hao Hu, Zhili Zhong, Xiaojun Guan, Yifu Zhao, Yunshi Wang, Lu Ye, Liang Ming, Liliang Riaz Rajoka, Muhammad Shahid He, Zhendan Wang, Yan Song, Xun Front Microbiol Microbiology In recent decades, infections caused by the opportunistic fungus Candida albicans have increased, especially in patients with immunodeficiency. In this study, we investigated the mechanism of action of sanguinarine (SAN) against C. albicans both in vitro and in vivo. SAN exhibited antifungal activity against C. albicans clinical isolates, with MICs in the range of 112.8–150.5 μM. Furthermore, scanning electron and transmission electron microscopy showed that SAN induced morphological changes as well as structure disruption in C. albicans cells, including masses of cellular debris, ruptured cell walls, and membrane deformation. Flow cytometry revealed that SAN could lead to cell membrane damage, and ergosterol content analysis indicated that SAN could cause ergosterol content reduction exceeding 90%. Further, we validated the efficacy of SAN against candidiasis caused by C. albicans in a murine model, and SAN significantly improved survival and reduced weight loss compared to vehicle. The treatment of 1.5 and 2.5 mg/kg/d SAN obviously reduced the fungal burden in the kidney. In addition, histopathological examination indicated that no fungal cells were observed in lung and kidney tissues after SAN treatment. Hence, this study suggests that SAN is a promising plant-derived compound for the development of an effective anticandidal agent. Frontiers Media S.A. 2022-06-15 /pmc/articles/PMC9240711/ /pubmed/35783394 http://dx.doi.org/10.3389/fmicb.2022.908461 Text en Copyright © 2022 Hu, Hu, Hu, Zhong, Guan, Zhao, Wang, Ye, Ming, Riaz Rajoka, He, Wang and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Hu, Ziwei Hu, Hao Hu, Zhili Zhong, Xiaojun Guan, Yifu Zhao, Yunshi Wang, Lu Ye, Liang Ming, Liliang Riaz Rajoka, Muhammad Shahid He, Zhendan Wang, Yan Song, Xun Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis |
title | Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis |
title_full | Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis |
title_fullStr | Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis |
title_full_unstemmed | Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis |
title_short | Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis |
title_sort | sanguinarine, isolated from macleaya cordata, exhibits potent antifungal efficacy against candida albicans through inhibiting ergosterol synthesis |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240711/ https://www.ncbi.nlm.nih.gov/pubmed/35783394 http://dx.doi.org/10.3389/fmicb.2022.908461 |
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