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Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis

In recent decades, infections caused by the opportunistic fungus Candida albicans have increased, especially in patients with immunodeficiency. In this study, we investigated the mechanism of action of sanguinarine (SAN) against C. albicans both in vitro and in vivo. SAN exhibited antifungal activit...

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Autores principales: Hu, Ziwei, Hu, Hao, Hu, Zhili, Zhong, Xiaojun, Guan, Yifu, Zhao, Yunshi, Wang, Lu, Ye, Liang, Ming, Liliang, Riaz Rajoka, Muhammad Shahid, He, Zhendan, Wang, Yan, Song, Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240711/
https://www.ncbi.nlm.nih.gov/pubmed/35783394
http://dx.doi.org/10.3389/fmicb.2022.908461
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author Hu, Ziwei
Hu, Hao
Hu, Zhili
Zhong, Xiaojun
Guan, Yifu
Zhao, Yunshi
Wang, Lu
Ye, Liang
Ming, Liliang
Riaz Rajoka, Muhammad Shahid
He, Zhendan
Wang, Yan
Song, Xun
author_facet Hu, Ziwei
Hu, Hao
Hu, Zhili
Zhong, Xiaojun
Guan, Yifu
Zhao, Yunshi
Wang, Lu
Ye, Liang
Ming, Liliang
Riaz Rajoka, Muhammad Shahid
He, Zhendan
Wang, Yan
Song, Xun
author_sort Hu, Ziwei
collection PubMed
description In recent decades, infections caused by the opportunistic fungus Candida albicans have increased, especially in patients with immunodeficiency. In this study, we investigated the mechanism of action of sanguinarine (SAN) against C. albicans both in vitro and in vivo. SAN exhibited antifungal activity against C. albicans clinical isolates, with MICs in the range of 112.8–150.5 μM. Furthermore, scanning electron and transmission electron microscopy showed that SAN induced morphological changes as well as structure disruption in C. albicans cells, including masses of cellular debris, ruptured cell walls, and membrane deformation. Flow cytometry revealed that SAN could lead to cell membrane damage, and ergosterol content analysis indicated that SAN could cause ergosterol content reduction exceeding 90%. Further, we validated the efficacy of SAN against candidiasis caused by C. albicans in a murine model, and SAN significantly improved survival and reduced weight loss compared to vehicle. The treatment of 1.5 and 2.5 mg/kg/d SAN obviously reduced the fungal burden in the kidney. In addition, histopathological examination indicated that no fungal cells were observed in lung and kidney tissues after SAN treatment. Hence, this study suggests that SAN is a promising plant-derived compound for the development of an effective anticandidal agent.
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spelling pubmed-92407112022-06-30 Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis Hu, Ziwei Hu, Hao Hu, Zhili Zhong, Xiaojun Guan, Yifu Zhao, Yunshi Wang, Lu Ye, Liang Ming, Liliang Riaz Rajoka, Muhammad Shahid He, Zhendan Wang, Yan Song, Xun Front Microbiol Microbiology In recent decades, infections caused by the opportunistic fungus Candida albicans have increased, especially in patients with immunodeficiency. In this study, we investigated the mechanism of action of sanguinarine (SAN) against C. albicans both in vitro and in vivo. SAN exhibited antifungal activity against C. albicans clinical isolates, with MICs in the range of 112.8–150.5 μM. Furthermore, scanning electron and transmission electron microscopy showed that SAN induced morphological changes as well as structure disruption in C. albicans cells, including masses of cellular debris, ruptured cell walls, and membrane deformation. Flow cytometry revealed that SAN could lead to cell membrane damage, and ergosterol content analysis indicated that SAN could cause ergosterol content reduction exceeding 90%. Further, we validated the efficacy of SAN against candidiasis caused by C. albicans in a murine model, and SAN significantly improved survival and reduced weight loss compared to vehicle. The treatment of 1.5 and 2.5 mg/kg/d SAN obviously reduced the fungal burden in the kidney. In addition, histopathological examination indicated that no fungal cells were observed in lung and kidney tissues after SAN treatment. Hence, this study suggests that SAN is a promising plant-derived compound for the development of an effective anticandidal agent. Frontiers Media S.A. 2022-06-15 /pmc/articles/PMC9240711/ /pubmed/35783394 http://dx.doi.org/10.3389/fmicb.2022.908461 Text en Copyright © 2022 Hu, Hu, Hu, Zhong, Guan, Zhao, Wang, Ye, Ming, Riaz Rajoka, He, Wang and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Hu, Ziwei
Hu, Hao
Hu, Zhili
Zhong, Xiaojun
Guan, Yifu
Zhao, Yunshi
Wang, Lu
Ye, Liang
Ming, Liliang
Riaz Rajoka, Muhammad Shahid
He, Zhendan
Wang, Yan
Song, Xun
Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
title Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
title_full Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
title_fullStr Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
title_full_unstemmed Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
title_short Sanguinarine, Isolated From Macleaya cordata, Exhibits Potent Antifungal Efficacy Against Candida albicans Through Inhibiting Ergosterol Synthesis
title_sort sanguinarine, isolated from macleaya cordata, exhibits potent antifungal efficacy against candida albicans through inhibiting ergosterol synthesis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240711/
https://www.ncbi.nlm.nih.gov/pubmed/35783394
http://dx.doi.org/10.3389/fmicb.2022.908461
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