EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle

N6-methyladenosine (m(6)A) mediates various biological processes by affecting RNA stability, splicing, and translational efficiency. The roles of m(6)A modification in Epstein-Barr virus (EBV) infection in the lytic phase are unclear. Here, knockout of the m(6)A methyltransferase, N6-methyladenosine...

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Autores principales: Yanagi, Yusuke, Watanabe, Takahiro, Hara, Yuya, Sato, Yoshitaka, Kimura, Hiroshi, Murata, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240777/
https://www.ncbi.nlm.nih.gov/pubmed/35783391
http://dx.doi.org/10.3389/fmicb.2022.870816
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author Yanagi, Yusuke
Watanabe, Takahiro
Hara, Yuya
Sato, Yoshitaka
Kimura, Hiroshi
Murata, Takayuki
author_facet Yanagi, Yusuke
Watanabe, Takahiro
Hara, Yuya
Sato, Yoshitaka
Kimura, Hiroshi
Murata, Takayuki
author_sort Yanagi, Yusuke
collection PubMed
description N6-methyladenosine (m(6)A) mediates various biological processes by affecting RNA stability, splicing, and translational efficiency. The roles of m(6)A modification in Epstein-Barr virus (EBV) infection in the lytic phase are unclear. Here, knockout of the m(6)A methyltransferase, N6-methyladenosine methyltransferase-like 3 (METTL3), or inhibition of methylation by DAA or UZH1a decreased the expression of viral lytic proteins and reduced progeny virion production. Interestingly, cell growth and viability were decreased by induction of the lytic cycle in METTL3-knockout or inhibitor-treated cells. Apoptosis was induced in those conditions possibly because of a decreased level of the anti-apoptotic viral protein, BHRF1. Therefore, m(6)A shows potential as a target of lytic induction therapy for EBV-associated cancers, including Burkitt lymphoma.
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spelling pubmed-92407772022-06-30 EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle Yanagi, Yusuke Watanabe, Takahiro Hara, Yuya Sato, Yoshitaka Kimura, Hiroshi Murata, Takayuki Front Microbiol Microbiology N6-methyladenosine (m(6)A) mediates various biological processes by affecting RNA stability, splicing, and translational efficiency. The roles of m(6)A modification in Epstein-Barr virus (EBV) infection in the lytic phase are unclear. Here, knockout of the m(6)A methyltransferase, N6-methyladenosine methyltransferase-like 3 (METTL3), or inhibition of methylation by DAA or UZH1a decreased the expression of viral lytic proteins and reduced progeny virion production. Interestingly, cell growth and viability were decreased by induction of the lytic cycle in METTL3-knockout or inhibitor-treated cells. Apoptosis was induced in those conditions possibly because of a decreased level of the anti-apoptotic viral protein, BHRF1. Therefore, m(6)A shows potential as a target of lytic induction therapy for EBV-associated cancers, including Burkitt lymphoma. Frontiers Media S.A. 2022-06-15 /pmc/articles/PMC9240777/ /pubmed/35783391 http://dx.doi.org/10.3389/fmicb.2022.870816 Text en Copyright © 2022 Yanagi, Watanabe, Hara, Sato, Kimura and Murata. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yanagi, Yusuke
Watanabe, Takahiro
Hara, Yuya
Sato, Yoshitaka
Kimura, Hiroshi
Murata, Takayuki
EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle
title EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle
title_full EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle
title_fullStr EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle
title_full_unstemmed EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle
title_short EBV Exploits RNA m(6)A Modification to Promote Cell Survival and Progeny Virus Production During Lytic Cycle
title_sort ebv exploits rna m(6)a modification to promote cell survival and progeny virus production during lytic cycle
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240777/
https://www.ncbi.nlm.nih.gov/pubmed/35783391
http://dx.doi.org/10.3389/fmicb.2022.870816
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