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Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection
Chikungunya virus (CHIKV) is a single-stranded positive RNA virus that belongs to the genus Alphavirus and is transmitted to humans by infected Aedes aegypti and Aedes albopictus bites. In humans, CHIKV usually causes painful symptoms during acute and chronic stages of infection. Conversely, virus–v...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240781/ https://www.ncbi.nlm.nih.gov/pubmed/35782121 http://dx.doi.org/10.3389/fcimb.2022.920425 |
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author | Vasconcellos, Anna Fernanda Melo, Reynaldo Magalhães Mandacaru, Samuel Coelho de Oliveira, Lucas Silva de Oliveira, Athos Silva Moraes, Emily Caroline dos Santos Trugilho, Monique Ramos de Oliveira Ricart, Carlos André Ornelas Báo, Sônia Nair Resende, Renato Oliveira Charneau, Sébastien |
author_facet | Vasconcellos, Anna Fernanda Melo, Reynaldo Magalhães Mandacaru, Samuel Coelho de Oliveira, Lucas Silva de Oliveira, Athos Silva Moraes, Emily Caroline dos Santos Trugilho, Monique Ramos de Oliveira Ricart, Carlos André Ornelas Báo, Sônia Nair Resende, Renato Oliveira Charneau, Sébastien |
author_sort | Vasconcellos, Anna Fernanda |
collection | PubMed |
description | Chikungunya virus (CHIKV) is a single-stranded positive RNA virus that belongs to the genus Alphavirus and is transmitted to humans by infected Aedes aegypti and Aedes albopictus bites. In humans, CHIKV usually causes painful symptoms during acute and chronic stages of infection. Conversely, virus–vector interaction does not disturb the mosquito’s fitness, allowing a persistent infection. Herein, we studied CHIKV infection of Ae. aegypti Aag-2 cells (multiplicity of infection (MOI) of 0.1) for 48 h through label-free quantitative proteomic analysis and transmission electron microscopy (TEM). TEM images showed a high load of intracellular viral cargo at 48 h postinfection (hpi), as well as an unusual elongated mitochondria morphology that might indicate a mitochondrial imbalance. Proteome analysis revealed 196 regulated protein groups upon infection, which are related to protein synthesis, energy metabolism, signaling pathways, and apoptosis. These Aag-2 proteins regulated during CHIKV infection might have roles in antiviral and/or proviral mechanisms and the balance between viral propagation and the survival of host cells, possibly leading to the persistent infection. |
format | Online Article Text |
id | pubmed-9240781 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-92407812022-06-30 Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection Vasconcellos, Anna Fernanda Melo, Reynaldo Magalhães Mandacaru, Samuel Coelho de Oliveira, Lucas Silva de Oliveira, Athos Silva Moraes, Emily Caroline dos Santos Trugilho, Monique Ramos de Oliveira Ricart, Carlos André Ornelas Báo, Sônia Nair Resende, Renato Oliveira Charneau, Sébastien Front Cell Infect Microbiol Cellular and Infection Microbiology Chikungunya virus (CHIKV) is a single-stranded positive RNA virus that belongs to the genus Alphavirus and is transmitted to humans by infected Aedes aegypti and Aedes albopictus bites. In humans, CHIKV usually causes painful symptoms during acute and chronic stages of infection. Conversely, virus–vector interaction does not disturb the mosquito’s fitness, allowing a persistent infection. Herein, we studied CHIKV infection of Ae. aegypti Aag-2 cells (multiplicity of infection (MOI) of 0.1) for 48 h through label-free quantitative proteomic analysis and transmission electron microscopy (TEM). TEM images showed a high load of intracellular viral cargo at 48 h postinfection (hpi), as well as an unusual elongated mitochondria morphology that might indicate a mitochondrial imbalance. Proteome analysis revealed 196 regulated protein groups upon infection, which are related to protein synthesis, energy metabolism, signaling pathways, and apoptosis. These Aag-2 proteins regulated during CHIKV infection might have roles in antiviral and/or proviral mechanisms and the balance between viral propagation and the survival of host cells, possibly leading to the persistent infection. Frontiers Media S.A. 2022-06-15 /pmc/articles/PMC9240781/ /pubmed/35782121 http://dx.doi.org/10.3389/fcimb.2022.920425 Text en Copyright © 2022 Vasconcellos, Melo, Mandacaru, de Oliveira, de Oliveira, Moraes, Trugilho, Ricart, Báo, Resende and Charneau https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Vasconcellos, Anna Fernanda Melo, Reynaldo Magalhães Mandacaru, Samuel Coelho de Oliveira, Lucas Silva de Oliveira, Athos Silva Moraes, Emily Caroline dos Santos Trugilho, Monique Ramos de Oliveira Ricart, Carlos André Ornelas Báo, Sônia Nair Resende, Renato Oliveira Charneau, Sébastien Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection |
title |
Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection |
title_full |
Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection |
title_fullStr |
Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection |
title_full_unstemmed |
Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection |
title_short |
Aedes aegypti Aag-2 Cell Proteome Modulation in Response to Chikungunya Virus Infection |
title_sort | aedes aegypti aag-2 cell proteome modulation in response to chikungunya virus infection |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240781/ https://www.ncbi.nlm.nih.gov/pubmed/35782121 http://dx.doi.org/10.3389/fcimb.2022.920425 |
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