Cargando…

Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs

Conductive hydrogels are emerging as promising materials for bioelectronic applications as they minimize the mismatch between biological and electronic systems. We propose a strategy to bioprint biohybrid conductive bioinks based on decellularized extracellular matrix (dECM) and multiwalled carbon n...

Descripción completa

Detalles Bibliográficos
Autores principales: Sanjuan-Alberte, Paola, Whitehead, Charlie, Jones, Joshua N., Silva, João C., Carter, Nathan, Kellaway, Simon, Hague, Richard J.M., Cabral, Joaquim M.S., Ferreira, Frederico C., White, Lisa J., Rawson, Frankie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240791/
https://www.ncbi.nlm.nih.gov/pubmed/35784786
http://dx.doi.org/10.1016/j.isci.2022.104552
_version_ 1784737648094478336
author Sanjuan-Alberte, Paola
Whitehead, Charlie
Jones, Joshua N.
Silva, João C.
Carter, Nathan
Kellaway, Simon
Hague, Richard J.M.
Cabral, Joaquim M.S.
Ferreira, Frederico C.
White, Lisa J.
Rawson, Frankie J.
author_facet Sanjuan-Alberte, Paola
Whitehead, Charlie
Jones, Joshua N.
Silva, João C.
Carter, Nathan
Kellaway, Simon
Hague, Richard J.M.
Cabral, Joaquim M.S.
Ferreira, Frederico C.
White, Lisa J.
Rawson, Frankie J.
author_sort Sanjuan-Alberte, Paola
collection PubMed
description Conductive hydrogels are emerging as promising materials for bioelectronic applications as they minimize the mismatch between biological and electronic systems. We propose a strategy to bioprint biohybrid conductive bioinks based on decellularized extracellular matrix (dECM) and multiwalled carbon nanotubes. These inks contained conductive features and morphology of the dECM fibers. Electrical stimulation (ES) was applied to bioprinted structures containing human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs). It was observed that in the absence of external ES, the conductive properties of the materials can improve the contractile behavior of the hPSC-CMs, and this effect is enhanced under the application of external ES. Genetic markers indicated a trend toward a more mature state of the cells with upregulated calcium handling proteins and downregulation of calcium channels involved in the generation of pacemaking currents. These results demonstrate the potential of our strategy to manufacture conductive hydrogels in complex geometries for actuating purposes.
format Online
Article
Text
id pubmed-9240791
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92407912022-06-30 Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs Sanjuan-Alberte, Paola Whitehead, Charlie Jones, Joshua N. Silva, João C. Carter, Nathan Kellaway, Simon Hague, Richard J.M. Cabral, Joaquim M.S. Ferreira, Frederico C. White, Lisa J. Rawson, Frankie J. iScience Article Conductive hydrogels are emerging as promising materials for bioelectronic applications as they minimize the mismatch between biological and electronic systems. We propose a strategy to bioprint biohybrid conductive bioinks based on decellularized extracellular matrix (dECM) and multiwalled carbon nanotubes. These inks contained conductive features and morphology of the dECM fibers. Electrical stimulation (ES) was applied to bioprinted structures containing human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs). It was observed that in the absence of external ES, the conductive properties of the materials can improve the contractile behavior of the hPSC-CMs, and this effect is enhanced under the application of external ES. Genetic markers indicated a trend toward a more mature state of the cells with upregulated calcium handling proteins and downregulation of calcium channels involved in the generation of pacemaking currents. These results demonstrate the potential of our strategy to manufacture conductive hydrogels in complex geometries for actuating purposes. Elsevier 2022-06-07 /pmc/articles/PMC9240791/ /pubmed/35784786 http://dx.doi.org/10.1016/j.isci.2022.104552 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sanjuan-Alberte, Paola
Whitehead, Charlie
Jones, Joshua N.
Silva, João C.
Carter, Nathan
Kellaway, Simon
Hague, Richard J.M.
Cabral, Joaquim M.S.
Ferreira, Frederico C.
White, Lisa J.
Rawson, Frankie J.
Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
title Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
title_full Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
title_fullStr Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
title_full_unstemmed Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
title_short Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs
title_sort printing biohybrid materials for bioelectronic cardio-3d-cellular constructs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240791/
https://www.ncbi.nlm.nih.gov/pubmed/35784786
http://dx.doi.org/10.1016/j.isci.2022.104552
work_keys_str_mv AT sanjuanalbertepaola printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT whiteheadcharlie printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT jonesjoshuan printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT silvajoaoc printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT carternathan printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT kellawaysimon printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT haguerichardjm printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT cabraljoaquimms printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT ferreirafredericoc printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT whitelisaj printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs
AT rawsonfrankiej printingbiohybridmaterialsforbioelectroniccardio3dcellularconstructs