Cargando…

Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion

This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol...

Descripción completa

Detalles Bibliográficos
Autores principales: Pavlović, Neda, Mijalković, Jelena, Đorđević, Verica, Pecarski, Danijela, Bugarski, Branko, Knežević-Jugović, Zorica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240801/
https://www.ncbi.nlm.nih.gov/pubmed/35782959
http://dx.doi.org/10.1016/j.fochx.2022.100370
_version_ 1784737650668732416
author Pavlović, Neda
Mijalković, Jelena
Đorđević, Verica
Pecarski, Danijela
Bugarski, Branko
Knežević-Jugović, Zorica
author_facet Pavlović, Neda
Mijalković, Jelena
Đorđević, Verica
Pecarski, Danijela
Bugarski, Branko
Knežević-Jugović, Zorica
author_sort Pavlović, Neda
collection PubMed
description This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol, and application of ultrasonication. Achieved results revealed that ultrasonication, together with cholesterol addition into phospholipid layers, improved the stability of nanoliposomes, and a maximum EE value of 60.5 % was obtained. Average size of peptide-loaded nanoliposomes was found to be from 191.1 to 286.7 nm, with a ζ potential of −25.5 to −34.6 mV, and a polydispersity index of 0.250–0.390. Ultrasound-assisted encapsulation process did not lead to a decrease in the antioxidant activity of the trapped peptides. FTIR has indicated an effective hydrophobic interaction between phosphatidylcholine and hydrolysate peptides. TEM and SEM have confirmed the spherical nanocarrier structure and unilamelarity. Prolonged gastrointestinal release and stability of peptides have been enabled by liposome nanocarriers.
format Online
Article
Text
id pubmed-9240801
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92408012022-06-30 Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion Pavlović, Neda Mijalković, Jelena Đorđević, Verica Pecarski, Danijela Bugarski, Branko Knežević-Jugović, Zorica Food Chem X Article This study presents the state-of-art research about the assembly of soy proteins in nanocarriers, liposomes, and its design includes different physicochemical strategies and approaches: two-step enzymatic hydrolysis of soy concentrate, hydrolysate encapsulation by using phospholipids and cholesterol, and application of ultrasonication. Achieved results revealed that ultrasonication, together with cholesterol addition into phospholipid layers, improved the stability of nanoliposomes, and a maximum EE value of 60.5 % was obtained. Average size of peptide-loaded nanoliposomes was found to be from 191.1 to 286.7 nm, with a ζ potential of −25.5 to −34.6 mV, and a polydispersity index of 0.250–0.390. Ultrasound-assisted encapsulation process did not lead to a decrease in the antioxidant activity of the trapped peptides. FTIR has indicated an effective hydrophobic interaction between phosphatidylcholine and hydrolysate peptides. TEM and SEM have confirmed the spherical nanocarrier structure and unilamelarity. Prolonged gastrointestinal release and stability of peptides have been enabled by liposome nanocarriers. Elsevier 2022-06-18 /pmc/articles/PMC9240801/ /pubmed/35782959 http://dx.doi.org/10.1016/j.fochx.2022.100370 Text en © 2022 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Pavlović, Neda
Mijalković, Jelena
Đorđević, Verica
Pecarski, Danijela
Bugarski, Branko
Knežević-Jugović, Zorica
Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
title Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
title_full Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
title_fullStr Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
title_full_unstemmed Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
title_short Ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: Process optimization, vesicle characteristics and in vitro digestion
title_sort ultrasonication for production of nanoliposomes with encapsulated soy protein concentrate hydrolysate: process optimization, vesicle characteristics and in vitro digestion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240801/
https://www.ncbi.nlm.nih.gov/pubmed/35782959
http://dx.doi.org/10.1016/j.fochx.2022.100370
work_keys_str_mv AT pavlovicneda ultrasonicationforproductionofnanoliposomeswithencapsulatedsoyproteinconcentratehydrolysateprocessoptimizationvesiclecharacteristicsandinvitrodigestion
AT mijalkovicjelena ultrasonicationforproductionofnanoliposomeswithencapsulatedsoyproteinconcentratehydrolysateprocessoptimizationvesiclecharacteristicsandinvitrodigestion
AT đorđevicverica ultrasonicationforproductionofnanoliposomeswithencapsulatedsoyproteinconcentratehydrolysateprocessoptimizationvesiclecharacteristicsandinvitrodigestion
AT pecarskidanijela ultrasonicationforproductionofnanoliposomeswithencapsulatedsoyproteinconcentratehydrolysateprocessoptimizationvesiclecharacteristicsandinvitrodigestion
AT bugarskibranko ultrasonicationforproductionofnanoliposomeswithencapsulatedsoyproteinconcentratehydrolysateprocessoptimizationvesiclecharacteristicsandinvitrodigestion
AT knezevicjugoviczorica ultrasonicationforproductionofnanoliposomeswithencapsulatedsoyproteinconcentratehydrolysateprocessoptimizationvesiclecharacteristicsandinvitrodigestion