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Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice

Aging causes astrocyte morphological degeneration and functional deficiency, which impairs neuronal functions. Until now, whether age-induced neuronal deficiency could be alleviated by engraftment of glial progenitor cell (GPC) derived astrocytes remained unknown. In the current study, GPCs were gen...

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Autores principales: Yang, Zhiqi, Gong, Mingyue, Jian, Tingliang, Li, Jin, Yang, Chuanyan, Ma, Qinlong, Deng, Ping, Wang, Yuxia, Huang, Mingzhu, Wang, Haoyu, Yang, Shaofan, Chen, Xiaowei, Yu, Zhengping, Wang, Manxia, Chen, Chunhai, Zhang, Kuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241208/
https://www.ncbi.nlm.nih.gov/pubmed/35765112
http://dx.doi.org/10.1186/s13287-022-02959-0
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author Yang, Zhiqi
Gong, Mingyue
Jian, Tingliang
Li, Jin
Yang, Chuanyan
Ma, Qinlong
Deng, Ping
Wang, Yuxia
Huang, Mingzhu
Wang, Haoyu
Yang, Shaofan
Chen, Xiaowei
Yu, Zhengping
Wang, Manxia
Chen, Chunhai
Zhang, Kuan
author_facet Yang, Zhiqi
Gong, Mingyue
Jian, Tingliang
Li, Jin
Yang, Chuanyan
Ma, Qinlong
Deng, Ping
Wang, Yuxia
Huang, Mingzhu
Wang, Haoyu
Yang, Shaofan
Chen, Xiaowei
Yu, Zhengping
Wang, Manxia
Chen, Chunhai
Zhang, Kuan
author_sort Yang, Zhiqi
collection PubMed
description Aging causes astrocyte morphological degeneration and functional deficiency, which impairs neuronal functions. Until now, whether age-induced neuronal deficiency could be alleviated by engraftment of glial progenitor cell (GPC) derived astrocytes remained unknown. In the current study, GPCs were generated from embryonic cortical neural stem cells in vitro and transplanted into the brains of aged mice. Their integration and intervention effects in the aged brain were examined 12 months after transplantation. Results indicated that these in-vitro-generated GPC-derived astrocytes possessed normal functional properties. After transplantation they could migrate, differentiate, achieve long-term integration, and maintain much younger morphology in the aged brain. Additionally, these GPC-derived astrocytes established endfeet expressing aquaporin-4 (AQP4) and ameliorate AQP4 polarization in the aged neocortex. More importantly, age-dependent sensory response degeneration was reversed by GPC transplantation. This work demonstrates that rejuvenation of the astrocyte niche is a promising treatment to prevent age-induced degradation of neuronal and behavioral functions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-022-02959-0.
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spelling pubmed-92412082022-06-30 Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice Yang, Zhiqi Gong, Mingyue Jian, Tingliang Li, Jin Yang, Chuanyan Ma, Qinlong Deng, Ping Wang, Yuxia Huang, Mingzhu Wang, Haoyu Yang, Shaofan Chen, Xiaowei Yu, Zhengping Wang, Manxia Chen, Chunhai Zhang, Kuan Stem Cell Res Ther Short Report Aging causes astrocyte morphological degeneration and functional deficiency, which impairs neuronal functions. Until now, whether age-induced neuronal deficiency could be alleviated by engraftment of glial progenitor cell (GPC) derived astrocytes remained unknown. In the current study, GPCs were generated from embryonic cortical neural stem cells in vitro and transplanted into the brains of aged mice. Their integration and intervention effects in the aged brain were examined 12 months after transplantation. Results indicated that these in-vitro-generated GPC-derived astrocytes possessed normal functional properties. After transplantation they could migrate, differentiate, achieve long-term integration, and maintain much younger morphology in the aged brain. Additionally, these GPC-derived astrocytes established endfeet expressing aquaporin-4 (AQP4) and ameliorate AQP4 polarization in the aged neocortex. More importantly, age-dependent sensory response degeneration was reversed by GPC transplantation. This work demonstrates that rejuvenation of the astrocyte niche is a promising treatment to prevent age-induced degradation of neuronal and behavioral functions. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-022-02959-0. BioMed Central 2022-06-28 /pmc/articles/PMC9241208/ /pubmed/35765112 http://dx.doi.org/10.1186/s13287-022-02959-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Yang, Zhiqi
Gong, Mingyue
Jian, Tingliang
Li, Jin
Yang, Chuanyan
Ma, Qinlong
Deng, Ping
Wang, Yuxia
Huang, Mingzhu
Wang, Haoyu
Yang, Shaofan
Chen, Xiaowei
Yu, Zhengping
Wang, Manxia
Chen, Chunhai
Zhang, Kuan
Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
title Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
title_full Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
title_fullStr Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
title_full_unstemmed Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
title_short Engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
title_sort engrafted glial progenitor cells yield long-term integration and sensory improvement in aged mice
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241208/
https://www.ncbi.nlm.nih.gov/pubmed/35765112
http://dx.doi.org/10.1186/s13287-022-02959-0
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