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The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications

There is growing evidence that post‐transcriptional RNA modifications are highly dynamic and can be used to improve crop production. Although more than 172 unique types of RNA modifications have been identified throughout the kingdom of life, we are yet to leverage upon the understanding to optimize...

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Autores principales: Ramakrishnan, Muthusamy, Rajan, K. Shanmugha, Mullasseri, Sileesh, Palakkal, Sarin, Kalpana, Krishnan, Sharma, Anket, Zhou, Mingbing, Vinod, Kunnummal Kurungara, Ramasamy, Subbiah, Wei, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241379/
https://www.ncbi.nlm.nih.gov/pubmed/35445501
http://dx.doi.org/10.1111/pbi.13829
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author Ramakrishnan, Muthusamy
Rajan, K. Shanmugha
Mullasseri, Sileesh
Palakkal, Sarin
Kalpana, Krishnan
Sharma, Anket
Zhou, Mingbing
Vinod, Kunnummal Kurungara
Ramasamy, Subbiah
Wei, Qiang
author_facet Ramakrishnan, Muthusamy
Rajan, K. Shanmugha
Mullasseri, Sileesh
Palakkal, Sarin
Kalpana, Krishnan
Sharma, Anket
Zhou, Mingbing
Vinod, Kunnummal Kurungara
Ramasamy, Subbiah
Wei, Qiang
author_sort Ramakrishnan, Muthusamy
collection PubMed
description There is growing evidence that post‐transcriptional RNA modifications are highly dynamic and can be used to improve crop production. Although more than 172 unique types of RNA modifications have been identified throughout the kingdom of life, we are yet to leverage upon the understanding to optimize RNA modifications in crops to improve productivity. The contributions of internal mRNA modifications such as N6‐methyladenosine (m(6)A) and 5‐methylcytosine (m(5)C) methylations to embryonic development, root development, leaf morphogenesis, flowering, fruit ripening and stress response are sufficiently known, but the roles of the two most abundant RNA modifications, pseudouridine (Ψ) and 2′‐O‐methylation (Nm), in the cell remain unclear due to insufficient advances in high‐throughput technologies in plant development. Therefore, in this review, we discuss the latest methods and insights gained in mapping internal Ψ and Nm and their unique properties in plants and other organisms. In addition, we discuss the limitations that remain in high‐throughput technologies for qualitative and quantitative mapping of these RNA modifications and highlight future challenges in regulating the plant epitranscriptome.
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spelling pubmed-92413792022-07-01 The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications Ramakrishnan, Muthusamy Rajan, K. Shanmugha Mullasseri, Sileesh Palakkal, Sarin Kalpana, Krishnan Sharma, Anket Zhou, Mingbing Vinod, Kunnummal Kurungara Ramasamy, Subbiah Wei, Qiang Plant Biotechnol J Review There is growing evidence that post‐transcriptional RNA modifications are highly dynamic and can be used to improve crop production. Although more than 172 unique types of RNA modifications have been identified throughout the kingdom of life, we are yet to leverage upon the understanding to optimize RNA modifications in crops to improve productivity. The contributions of internal mRNA modifications such as N6‐methyladenosine (m(6)A) and 5‐methylcytosine (m(5)C) methylations to embryonic development, root development, leaf morphogenesis, flowering, fruit ripening and stress response are sufficiently known, but the roles of the two most abundant RNA modifications, pseudouridine (Ψ) and 2′‐O‐methylation (Nm), in the cell remain unclear due to insufficient advances in high‐throughput technologies in plant development. Therefore, in this review, we discuss the latest methods and insights gained in mapping internal Ψ and Nm and their unique properties in plants and other organisms. In addition, we discuss the limitations that remain in high‐throughput technologies for qualitative and quantitative mapping of these RNA modifications and highlight future challenges in regulating the plant epitranscriptome. John Wiley and Sons Inc. 2022-05-11 2022-07 /pmc/articles/PMC9241379/ /pubmed/35445501 http://dx.doi.org/10.1111/pbi.13829 Text en © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Review
Ramakrishnan, Muthusamy
Rajan, K. Shanmugha
Mullasseri, Sileesh
Palakkal, Sarin
Kalpana, Krishnan
Sharma, Anket
Zhou, Mingbing
Vinod, Kunnummal Kurungara
Ramasamy, Subbiah
Wei, Qiang
The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications
title The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications
title_full The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications
title_fullStr The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications
title_full_unstemmed The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications
title_short The plant epitranscriptome: revisiting pseudouridine and 2′‐O‐methyl RNA modifications
title_sort plant epitranscriptome: revisiting pseudouridine and 2′‐o‐methyl rna modifications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241379/
https://www.ncbi.nlm.nih.gov/pubmed/35445501
http://dx.doi.org/10.1111/pbi.13829
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