Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing

Downstream next-generation sequencing (NGS) of the syphilis spirochete Treponema pallidum subspecies pallidum (T. pallidum) is hindered by low bacterial loads and the overwhelming presence of background metagenomic DNA in clinical specimens. In this study, we investigated selective whole-genome ampl...

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Detalles Bibliográficos
Autores principales: Thurlow, Charles M., Joseph, Sandeep J., Ganova-Raeva, Lilia, Katz, Samantha S., Pereira, Lara, Chen, Cheng, Debra, Alyssa, Vilfort, Kendra, Workowski, Kimberly, Cohen, Stephanie E., Reno, Hilary, Sun, Yongcheng, Burroughs, Mark, Sheth, Mili, Chi, Kai-Hua, Danavall, Damien, Philip, Susan S., Cao, Weiping, Kersh, Ellen N., Pillay, Allan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241506/
https://www.ncbi.nlm.nih.gov/pubmed/35491834
http://dx.doi.org/10.1128/msphere.00009-22

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