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An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies

Shigellosis has been a menace to society for ages. The absence of an effective vaccine against Shigella, improper sanitation, and unhygienic use of food and water allow the disease to flourish. Shigella can also be transmitted via natural water bodies. In the absence of a good animal model, the actu...

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Autores principales: Howlader, Debaki R., Bhaumik, Ushasi, Halder, Prolay, Satpathy, Aishwarya, Sarkar, Sounak, Ghoshal, Mrinalini, Maiti, Suhrid, Withey, Jeffrey H., Mitobe, Jiro, Dutta, Shanta, Koley, Hemanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241715/
https://www.ncbi.nlm.nih.gov/pubmed/35604149
http://dx.doi.org/10.1128/spectrum.00347-22
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author Howlader, Debaki R.
Bhaumik, Ushasi
Halder, Prolay
Satpathy, Aishwarya
Sarkar, Sounak
Ghoshal, Mrinalini
Maiti, Suhrid
Withey, Jeffrey H.
Mitobe, Jiro
Dutta, Shanta
Koley, Hemanta
author_facet Howlader, Debaki R.
Bhaumik, Ushasi
Halder, Prolay
Satpathy, Aishwarya
Sarkar, Sounak
Ghoshal, Mrinalini
Maiti, Suhrid
Withey, Jeffrey H.
Mitobe, Jiro
Dutta, Shanta
Koley, Hemanta
author_sort Howlader, Debaki R.
collection PubMed
description Shigellosis has been a menace to society for ages. The absence of an effective vaccine against Shigella, improper sanitation, and unhygienic use of food and water allow the disease to flourish. Shigella can also be transmitted via natural water bodies. In the absence of a good animal model, the actual nature of pathogenesis and transmission remains unclear. Zebrafish larvae have previously been described as a model for Shigella pathogenesis. However, larval fish lack a mature intestinal microbiota and immune system. Here, the adult zebrafish was assessed as a potential model for Shigella pathogenesis. Their well-developed innate and adaptive immune responses mimic the mammalian immune system. Shigella showed a clear dose-, time-, and temperature-dependent colonization of the adult zebrafish gut. Efficacy of a three-dose immunization regime was tested using bath immunization with heat-killed trivalent Shigella immunogen. The present study demonstrates the efficacy of an adult zebrafish model for pathogenesis, transmission, and vaccine efficacy studies. IMPORTANCE Shigellosis is a diarrheal disease that is prevalent in developing countries and especially dangerous in young children. Currently, animal models for shigellosis are unable to model some aspects of the infectious cycle. Here, we describe a new shigellosis model in adult zebrafish, an increasingly common model organism for studying bacterial pathogens. The zebrafish model can be used to study Shigella colonization, transmission, and immune responses, as well as test vaccine efficacy.
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spelling pubmed-92417152022-06-30 An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies Howlader, Debaki R. Bhaumik, Ushasi Halder, Prolay Satpathy, Aishwarya Sarkar, Sounak Ghoshal, Mrinalini Maiti, Suhrid Withey, Jeffrey H. Mitobe, Jiro Dutta, Shanta Koley, Hemanta Microbiol Spectr Research Article Shigellosis has been a menace to society for ages. The absence of an effective vaccine against Shigella, improper sanitation, and unhygienic use of food and water allow the disease to flourish. Shigella can also be transmitted via natural water bodies. In the absence of a good animal model, the actual nature of pathogenesis and transmission remains unclear. Zebrafish larvae have previously been described as a model for Shigella pathogenesis. However, larval fish lack a mature intestinal microbiota and immune system. Here, the adult zebrafish was assessed as a potential model for Shigella pathogenesis. Their well-developed innate and adaptive immune responses mimic the mammalian immune system. Shigella showed a clear dose-, time-, and temperature-dependent colonization of the adult zebrafish gut. Efficacy of a three-dose immunization regime was tested using bath immunization with heat-killed trivalent Shigella immunogen. The present study demonstrates the efficacy of an adult zebrafish model for pathogenesis, transmission, and vaccine efficacy studies. IMPORTANCE Shigellosis is a diarrheal disease that is prevalent in developing countries and especially dangerous in young children. Currently, animal models for shigellosis are unable to model some aspects of the infectious cycle. Here, we describe a new shigellosis model in adult zebrafish, an increasingly common model organism for studying bacterial pathogens. The zebrafish model can be used to study Shigella colonization, transmission, and immune responses, as well as test vaccine efficacy. American Society for Microbiology 2022-05-23 /pmc/articles/PMC9241715/ /pubmed/35604149 http://dx.doi.org/10.1128/spectrum.00347-22 Text en Copyright © 2022 Howlader et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Howlader, Debaki R.
Bhaumik, Ushasi
Halder, Prolay
Satpathy, Aishwarya
Sarkar, Sounak
Ghoshal, Mrinalini
Maiti, Suhrid
Withey, Jeffrey H.
Mitobe, Jiro
Dutta, Shanta
Koley, Hemanta
An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies
title An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies
title_full An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies
title_fullStr An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies
title_full_unstemmed An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies
title_short An Experimental Adult Zebrafish Model for Shigella Pathogenesis, Transmission, and Vaccine Efficacy Studies
title_sort experimental adult zebrafish model for shigella pathogenesis, transmission, and vaccine efficacy studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241715/
https://www.ncbi.nlm.nih.gov/pubmed/35604149
http://dx.doi.org/10.1128/spectrum.00347-22
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