Rapid Hypothesis Testing in Candida albicans Clinical Isolates Using a Cloning-Free, Modular, and Recyclable System for CRISPR-Cas9 Mediated Mutant and Revertant Construction

As increasing evidence emerges that interstrain genetic diversity among Candida albicans clinical isolates underpins phenotypic variation compared to the reference isolate SC5314, new genetic tools are required to interrogate gene function across strain backgrounds. Here, the SAT1-flipper plasmid wa...

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Detalles Bibliográficos
Autores principales: Liu, Junyan, Vogel, Amanda K., Miao, Jian, Carnahan, Jennifer A., Lowes, David J., Rybak, Jeffrey M., Peters, Brian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241802/
https://www.ncbi.nlm.nih.gov/pubmed/35612314
http://dx.doi.org/10.1128/spectrum.02630-21

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