Development of a gRNA Expression and Processing Platform for Efficient CRISPR-Cas9-Based Gene Editing and Gene Silencing in Candida tropicalis

Candida tropicalis, a nonmodel diploid microbe, has been applied in industry as a chassis cell. Metabolic engineering of C. tropicalis is challenging due to a lack of gene editing and regulation tools. Here, we report a tRNA:guide RNA (gRNA) platform for boosting gene editing and silencing efficienc...

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Detalles Bibliográficos
Autores principales: Li, Yujie, Zhang, Lihua, Yang, Haiquan, Xia, Yuanyuan, Liu, Liming, Chen, Xianzhong, Shen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241840/
https://www.ncbi.nlm.nih.gov/pubmed/35543560
http://dx.doi.org/10.1128/spectrum.00059-22

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