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Successful Direct Whole Genome Sequencing and Revivification of Freeze-Dried Nontuberculous Mycobacteria after More than Half a Century of Storage

In this study, 28 “historical” clinical freeze-dried nontuberculous mycobacterial isolates collected from 1948 to 1957, were analyzed by investigating their viability and performing whole genome sequencing (WGS) on DNA extracted (i) directly from freeze-dried cells versus (ii) after culturing, to de...

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Detalles Bibliográficos
Autores principales: Iversen, Xenia Emilie Sinding, Norman, Anders, Folkvardsen, Dorte Bek, Svensson, Erik, Rasmussen, Erik Michael, Lillebaek, Troels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241853/
https://www.ncbi.nlm.nih.gov/pubmed/35587637
http://dx.doi.org/10.1128/spectrum.00310-22