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Directed evolution of a cyclodipeptide synthase with new activities via label-free mass spectrometric screening
Directed evolution is a powerful approach to engineer enzymes via iterative creation and screening of variant libraries. However, assay development for high-throughput mutant screening remains challenging, particularly for new catalytic activities. Mass spectrometry (MS) analysis is label-free and w...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society of Chemistry
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241961/ https://www.ncbi.nlm.nih.gov/pubmed/35872818 http://dx.doi.org/10.1039/d2sc01637k |