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Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies

Aging-induced memory impairment is closely associated with oxidative stress. D-Galactose (D-gal) evokes severe oxidative stress and mimics normal aging in animals. Curcumin, a natural flavonoid, has potent antioxidant and anti-aging properties. There are several proteins like glutathione S-transfera...

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Autores principales: Rahman, Md. Ashrafur, Shuvo, Arif Anzum, Bepari, Asim Kumar, Hasan Apu, Mehedi, Shill, Manik Chandra, Hossain, Murad, Uddin, Mohammed, Islam, Md. Rabiul, Bakshi, Monjurul Kader, Hasan, Javed, Rahman, Atiqur, Rahman, Ghazi Mohammad Sayedur, Reza, Hasan Mahmud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242463/
https://www.ncbi.nlm.nih.gov/pubmed/35767571
http://dx.doi.org/10.1371/journal.pone.0270123
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author Rahman, Md. Ashrafur
Shuvo, Arif Anzum
Bepari, Asim Kumar
Hasan Apu, Mehedi
Shill, Manik Chandra
Hossain, Murad
Uddin, Mohammed
Islam, Md. Rabiul
Bakshi, Monjurul Kader
Hasan, Javed
Rahman, Atiqur
Rahman, Ghazi Mohammad Sayedur
Reza, Hasan Mahmud
author_facet Rahman, Md. Ashrafur
Shuvo, Arif Anzum
Bepari, Asim Kumar
Hasan Apu, Mehedi
Shill, Manik Chandra
Hossain, Murad
Uddin, Mohammed
Islam, Md. Rabiul
Bakshi, Monjurul Kader
Hasan, Javed
Rahman, Atiqur
Rahman, Ghazi Mohammad Sayedur
Reza, Hasan Mahmud
author_sort Rahman, Md. Ashrafur
collection PubMed
description Aging-induced memory impairment is closely associated with oxidative stress. D-Galactose (D-gal) evokes severe oxidative stress and mimics normal aging in animals. Curcumin, a natural flavonoid, has potent antioxidant and anti-aging properties. There are several proteins like glutathione S-transferase A1 (GSTA1), glutathione S-transferase omega-1 (GSTO1), kelch-like ECH-associated protein 1 (KEAP1), beta-secretase 1 (BACE1), and amine oxidase [flavin-containing] A (MAOA) are commonly involved in oxidative stress and aging. This study aimed to investigate the interaction of curcumin to these proteins and their subsequent effect on aging-associated memory impairment in two robust animal models: D-Gal and normal aged (NA) mice. The aging mice model was developed by administering D-gal intraperitoneally (i.p). Mice (n = 64) were divided into the eight groups (8 mice in each group): Vehicle, Curcumin-Control, D-gal (100mg/kg; i.p), Curcumin + D-gal, Astaxanthin (Ast) + D-gal, Normal Aged (NA), Curcumin (30mg/kg Orally) + NA, Ast (20mg/kg Orally) + NA. Retention and freezing memories were assessed by passive avoidance (PA) and contextual fear conditioning (CFC). Molecular docking was performed to predict curcumin binding with potential molecular targets. Curcumin significantly increased retention time (p < 0.05) and freezing response (p < 0.05) in PA and CFC, respectively. Curcumin profoundly ameliorated the levels of glutathione, superoxide dismutase, catalase, advanced oxidation protein products, nitric oxide, and lipid peroxidation in mice hippocampi. In silico studies revealed favorable binding energies of curcumin with GSTA1, GSTO1, KEAP1, BACE1, and MAOA. Curcumin improves retention and freezing memory in D-gal and nature-induced aging mice. Curcumin ameliorates the levels of oxidative stress biomarkers in mice. Anti-aging effects of curcumin could be attributed to, at least partially, the upregulation of antioxidant enzymes through binding with GSTA1, GSTO1, KEAP1, and inhibition of oxidative damage through binding with BACE1 and MAOA.
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spelling pubmed-92424632022-06-30 Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies Rahman, Md. Ashrafur Shuvo, Arif Anzum Bepari, Asim Kumar Hasan Apu, Mehedi Shill, Manik Chandra Hossain, Murad Uddin, Mohammed Islam, Md. Rabiul Bakshi, Monjurul Kader Hasan, Javed Rahman, Atiqur Rahman, Ghazi Mohammad Sayedur Reza, Hasan Mahmud PLoS One Research Article Aging-induced memory impairment is closely associated with oxidative stress. D-Galactose (D-gal) evokes severe oxidative stress and mimics normal aging in animals. Curcumin, a natural flavonoid, has potent antioxidant and anti-aging properties. There are several proteins like glutathione S-transferase A1 (GSTA1), glutathione S-transferase omega-1 (GSTO1), kelch-like ECH-associated protein 1 (KEAP1), beta-secretase 1 (BACE1), and amine oxidase [flavin-containing] A (MAOA) are commonly involved in oxidative stress and aging. This study aimed to investigate the interaction of curcumin to these proteins and their subsequent effect on aging-associated memory impairment in two robust animal models: D-Gal and normal aged (NA) mice. The aging mice model was developed by administering D-gal intraperitoneally (i.p). Mice (n = 64) were divided into the eight groups (8 mice in each group): Vehicle, Curcumin-Control, D-gal (100mg/kg; i.p), Curcumin + D-gal, Astaxanthin (Ast) + D-gal, Normal Aged (NA), Curcumin (30mg/kg Orally) + NA, Ast (20mg/kg Orally) + NA. Retention and freezing memories were assessed by passive avoidance (PA) and contextual fear conditioning (CFC). Molecular docking was performed to predict curcumin binding with potential molecular targets. Curcumin significantly increased retention time (p < 0.05) and freezing response (p < 0.05) in PA and CFC, respectively. Curcumin profoundly ameliorated the levels of glutathione, superoxide dismutase, catalase, advanced oxidation protein products, nitric oxide, and lipid peroxidation in mice hippocampi. In silico studies revealed favorable binding energies of curcumin with GSTA1, GSTO1, KEAP1, BACE1, and MAOA. Curcumin improves retention and freezing memory in D-gal and nature-induced aging mice. Curcumin ameliorates the levels of oxidative stress biomarkers in mice. Anti-aging effects of curcumin could be attributed to, at least partially, the upregulation of antioxidant enzymes through binding with GSTA1, GSTO1, KEAP1, and inhibition of oxidative damage through binding with BACE1 and MAOA. Public Library of Science 2022-06-29 /pmc/articles/PMC9242463/ /pubmed/35767571 http://dx.doi.org/10.1371/journal.pone.0270123 Text en © 2022 Rahman et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rahman, Md. Ashrafur
Shuvo, Arif Anzum
Bepari, Asim Kumar
Hasan Apu, Mehedi
Shill, Manik Chandra
Hossain, Murad
Uddin, Mohammed
Islam, Md. Rabiul
Bakshi, Monjurul Kader
Hasan, Javed
Rahman, Atiqur
Rahman, Ghazi Mohammad Sayedur
Reza, Hasan Mahmud
Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies
title Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies
title_full Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies
title_fullStr Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies
title_full_unstemmed Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies
title_short Curcumin improves D-galactose and normal-aging associated memory impairment in mice: In vivo and in silico-based studies
title_sort curcumin improves d-galactose and normal-aging associated memory impairment in mice: in vivo and in silico-based studies
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9242463/
https://www.ncbi.nlm.nih.gov/pubmed/35767571
http://dx.doi.org/10.1371/journal.pone.0270123
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